Placental peptide injection on human umbilical vein endothelial cell proliferation.docVIP

 PAGE \* MERGEFORMAT 9 Placental peptide injection on human umbilical vein endothelial cell proliferation [Abstract] Objective: To study the placental peptide injection on human umbilical vein endothelial cells (ECV-304) proliferation. Methods: The cell count and MTT method the effects of different dilutions of the role of the placenta 72 h after the peptide, ECV-304 cell proliferation. Simultaneously detected by immunohistochemistry in different dilutions of placental peptides on ECV-304 cells, proliferating cell nuclear antigen (PCNA) expression. Results: The dilution of the placenta certain peptides can promote the proliferation of ECV-304 cells, 25 ml / L can play a role, 200 ml / L the role of the most obvious. ECV-304 cells, the positive rate of PCNA expression and placental peptide dilution in a dose-related. Conclusion: Placental peptides can promote the in vitro cultured ECV-304 cell proliferation. [Keywords:] placenta; cell count; immunohistochemistry; placental polypeptide; endothelial cell [Abstract] Objective: To investigate the effects of placenta polypeptide on the proliferation of human umbilical vein endothelial cell (ECV-304) in vitro. Methods: ECV-304 cells were treated with different concentrations of placenta polypeptide, and the ECV-304 proliferation was observed with cytometry, and MTT in 72 h.Proliferating cell nuclear antigen (PCNA) was observed with immunohistochemistry method at the same time. Results: in the range of 25 ~ 400 ml / L, placenta polypeptide showed effect on ECV-304 proliferation in vitro in a dose-dependent manner and the maximal effect showed at 200 ml / L. Positive PCNA expression of ECV-304 correlated with placenta polypeptide concentrations used. Conclusion: Placenta polypeptide could promote the proliferation of ECV-304 in vitro. [Keywords:] placenta; cell count; immunohistochemistry; placents polypeptide; endothelial cell In recent years, as people gradually in-depth study of the placenta, placental processin