PLK-1 in acute leukemia cells and its significance.doc

 PAGE \* MERGEFORMAT 5 PLK-1 in acute leukemia cells and its significance Author: MAO Han-wen, Liu Li, Jian-Feng Zhou, SUN Han-ying, Xu Huizhen, Luo Xiao-hua [Abstract] In order to explore the acute leukemia cells plk-1 gene and its protein expression and significance, and a preliminary understanding of PLK-1 protein in acute leukemia cells in the distribution, by taking patients with acute leukemia, bone marrow benign hyperplasia and normal person’s bone marrow or peripheral blood mononuclear cells as object of study, application of RT-PCR, and flow cytometry detection of plk-1 mRNA and PLK-1 protein in acute leukemia cells, using fluorescence microscopy PLK-1 protein in the The distribution of acute leukemia cells. The results showed that: acute leukemia cells plk-1 at the gene expression levels and protein levels were significantly increased; fluorescent microscope examination showed that cell division and interphase PLK-1 protein in high concentration, the scattered evenly distributed in the acute leukemia cells in mitosis process, the sister chromatids to the cell poles when the curtain PLK-1 protein was mainly distributed in between the sister chromatids. The normal bone marrow cells and bone marrow cells in benign prostatic hyperplasia plk-1 at the gene level and protein level is almost not expressed. Conclusion: PLK-1 protein in acute leukemia cells may play an important role in abnormal proliferation and its expression correlated with the degree of malignancy. PLK-1 protein or its gene may be used as a new target for anti-tumor drugs can serve as a judge efficacy and prognosis of acute leukemia, one of the effective index. [Keywords:] Acute leukemia; plk-1 gene; PLK-1 protein; acute leukemia cells; gene expression Expression of plk-1 Gene in Acute Leukemia Patients and Its Significanc AbstractTo investigate expression of plk-1 gene and PLK-1 protein in acute leukemia patients and its clinical significance, and to observe the distribution of PLk-