POAG disease-causing gene MYOC mutant vector and in the COS7 cells.docVIP

 PAGE \* MERGEFORMAT 13 POAG disease-causing gene MYOC mutant vector and in the COS7 cells [Abstract] Objective: To construct Pro370Leu mutation type Myocilin (MYOC) gene eukaryotic expression plasmid, and expressed in COS  7 cells to examine the characteristics of mutant protein expression. Methods: pGEM  T  MYOC in MYOC plasmid as template, using PCR -mediated site-directed mutagenesis techniques, get Pro370Leu mutant MYOC gene (mutant MYOC, mMYOC), and subcloned into the eukaryotic expression vector pDsRed2  N1, commanding pDsRed2  N1  mMYOC recombinant expression plasmid,