Polycaprolactone electrospun fiber scaffold material on bone marrow stromal cell proliferation and differentiation of.docVIP

 PAGE \* MERGEFORMAT 7 Polycaprolactone electrospun fiber scaffold material on bone marrow stromal cell proliferation and differentiation of [Abstract] Objective: To observe the polycaprolactone (PCL) electrospun fiber scaffold material on bone marrow stromal cells (BMSCs) proliferation and differentiation of the characteristics of the impact assessment as a scaffold for bone tissue engineering application prospects. Methods: Rabbit BMSCs with the PCL electrospun fiber scaffolds co-cultured in the culture plate. using morphological observation, MTT method, and ALP detection BMSCs were detected in the PCL electrospun fiber surface adhesion, proliferation and differentiation. Results: BMSCs can be cultured in vitro In the PCL electrospun fiber surface of the normal adhesion and proliferation, and expression of high alkaline phosphatase activity. CONCLUSION: PCL electrospun fiber suitable as a scaffold material used in the organization of BMSCs as seed cells to build. [Keywords:] electrospun fiber; Polycaprolactone; bone marrow stromal cells 0 Introduction The ideal tissue engineering scaffold material requiring not only can be used as seed cells in vivo stent expansion and proliferation, but also should be able to become a growth factor, biologically active substances and genetic bio-carrier, to play a role in regulating cell function [1]. At present, the success of nearly one hundred kinds of polymers obtained by this technology was gradually applied to ultra-fine fiber membrane technology to enhance materials, textiles, optical sensors, medical delivery systems, as well as tissue engineering scaffold material preparation and many other fields [2]. This study designed PCL (polycaprolactone, PCL) electrospun fiber scaffold material for bone marrow stromal cells (bone marrow stromal cells, BMSCs) growth, proliferation and functional differentiation in vitro, preliminary study PCL electrospun fiber as tissue engineering scaffolds material application. 1 Materi