Porcine epidemic diarrhea virus M protein B cell epitope prediction analysis.doc

 PAGE \* MERGEFORMAT 10 Porcine epidemic diarrhea virus M protein B cell epitope prediction analysis Of: Wang Shen, high Shen Yang, Zhou Tiezhong, Bi Congming [Abstract] Objective To analyze the TGEV M protein B cell epitope, for the trial to determine TGEV M protein B cell epitope and the development of recombinant subunit vaccine TGEV basis; ways to TGEV M protein amino acid sequence-based, respectively Garnier- Robson, Chou-Fasman and M Karplus-Schulz method of protein secondary structure prediction, and the use of online TMHMM Server v2.0 software to analyze M protein transmembrane region; after, respectively, according to Kyte-Doolittle, Emini, and Jameson-Wolf program forecast TGEV M protein B cell epitope; results predicted results show that the TGEV M protein N-co @ - helical segments of 4; common -sheet section 13, respectively; the common areas were 7 corner ; flexible region 12; M protein transmembrane domain 3; conclusion TGEV M protein N-19 ~ 43,103 ~ 109,138 ~ 155,198 ~ 205,220 ~ 257 ~ 228 and 237 in or near section is likely to be dominant B cell epitope region. [Keywords:] TGEV; M protein; B cell epitope Abstract: Objective To identify the potential B cell epitopes of M protein of TGEV in theory, which is helpful for the development recombinant subunit vaccine of TGEV. Methods The secondary structure of M protein of TGEV and its transmembrane region were predicted by the methods of Garnier -Robson, Chou-Fasman, Karplus-Schulz and TMHMM Server v2.0 software online respectively, based on its amino acids sequence. And hydrophilicity plot, surface probability plot and antigenic index for M protein were analyzed by the methods of Kyte-Doolittle, Emini and Jameson-Wolf methods, respectively. Results There are 4 centers of @-helix, 13 centers of -sheet, 7 turn regions, 12 flexible regions, and 3 TMs in the M protein s N terminal, respectively.Conclusions Combined the previous results, the B cell epitopes for M protein of TGEV were predicte