Potassium iodide on experimental lead nephropathy protection and its effect on transforming growth factor β expression.docVIP

 PAGE \* MERGEFORMAT 13 Potassium iodide on experimental lead nephropathy protection and its effect on transforming growth factor β expression Author: Qiao Yufeng, Pangdong Zi, Rong Shan, Jiang Yunsheng [Abstract] Objective: Lead nephropathy of potassium iodide for protection, and from the protein and molecular level observed on transforming growth factor β (TGF β) expression. METHODS: SD rats 48 (180 ~ 220 g) to adapt of feeding 1 week, were randomly divided into two groups according to the different time points was divided into three sub-groups. APb, BPb, CPb for the lead-exposed group were given 0.5% lead acetate drinking, 2, 3-month feeding ; AKI, BKI, CKI group for the potassium iodide, 0.5% lead acetate given to drink while I fed potassium iodide (3 mg/100 g body weight), diluted with water for injection, orally once a day, times were 1,2,3 months All experimental group rats were roughly the same amount of drinking water per day. end of the experiment weighed, kidney weight; graphite furnace atomic absorption spectroscopy measurement of blood lead and renal lead content; immunohistochemical method to detect TGF β protein in kidney tissue expression, using RT PCR method to detect renal TGF β mRNA expression. Results: to 3 months, potassium iodide group and lead-exposed group body weight difference was significantly higher kidney weight / body weight ratio was significantly reduced; blood lead, kidney lead content was significantly reduced; and two months before the experiment no significant difference in renal tissue TGF β protein and mRNA expression in the two groups showed no significant Conclusion: potassium iodide can antagonize the lead on rat growth and development, and removing lead role, but has little effect on the TGF β. [Key Words] KI; lead nephropathy; transforming growth factor β Abstract Objective: To investigate the protective effect of potassium iodide on experimental lead nephropathy, and its influence on the expression of tran