Poisson callus induction_0.docVIP

Poisson callus induction [Abstract] Objective To investigate factors that affect the Poisson callus induction, callus culture method for establishing Poisson. Methods of tissue culture, comparison of different explants, pH, temperature, type and proportion of plant growth substances on the Poisson callus induction. results in MS medium, and leaves more than 80% induction rate, stem and root hard callus induction rate of nearly 10%; no significant difference between the different pH; optimum temperature for callus induction is 25 ℃; 6-BA 1.0 mg · L-1 + NAA 0.1 mg · L-1 induced the highest rate. Conclusion Poisson callus culture of leaf explants, suitable medium for the MS + 6-BA 1.0 mg · L-1 + NAA 0.1 mg · L-1 (pH 5.8), culture temperature is 25 ℃, light 12 h · d-1, light intensity 1200 Lx. [Keywords:] Poisson; callus; induction Poisson as sedum Poisson Orostachyd fimbriatus (Turcz.) Berger or later Red Poisson O. erudescens (Maxim.) Ohwi the whole plant. Are distributed throughout the country, was born in the roof tile cracks or rocks, but also known as the “house on the fairy.” Poisson clearing and detoxification, bleeding, dampness, swelling and other effects, for the treatment of hematemesis, epistaxis, Campylobacter, malaria, hemorrhoids, eczema, drug carbuncle, boils embolism [ 1]. As Poisson huge market demand, values #8203;#8203;are high, wild resources are limited, at present the medicinal raw materials, extracts and preparations of the raw material supply is totally dependent on wild resources, excessive excavation, so that shortage of resources and endangered wild Poisson extinction, in order to effectively protect their wildlife resources and sustainable development and utilization of callus induction on the Poisson was studied for the training of a Poisson source to provide the material basis for new drugs. 1 Materials and equipment 1.1 Poisson fresh plant material, collected from Xinjiang Yili, the Jiujiang University Medical College Depa