Placental biological characteristics of adherent cells.docVIP

 PAGE \* MERGEFORMAT 12 Placental biological characteristics of adherent cells [Keywords:] placenta; adherent cells; stem cells Biological characteristics of placentaderived adherent cells [Abstract] AIM: To isolate and culture human placentaderived adherent cells (hPDAC) in vitro and study their biological characteristics involving morphology, phenotype and differentiation. METHODS: Mononuclear cells were harvested after placenta tissue was digested by collagenase IV, seeded and cultured in lowglucose DMEM containing 100 mL / L FBS. Their surface markers were detected by flow cytometry; the growth curve was made; cells of 3 passages were induced to differentiate into osteoblasts with vitamin C, dexamethasone and sodium β glycerophosphate, and the induced cells were observed in the change of matrix mineralization with alizarin red staining; cells of 3 passages were induced to differentiate into lipocytes with dexamethasone and insulin, and the induced cells were observed with oil red O staining. RESULTS: By day 7-14, a few of adherent cells were observed and expanded the same as fibroblasts, and adherent cells were positive for CD29, CD44 and CD105, but negative for CD34, CD45, CD19. After 10day induction, alizarin red staining showed the calcium mineralization and after 7 d, oil red O staining revealed the appearance of fat drops. CONCLUSION: The hPDAC was confirmed to have the osteogenic and adipogenic potentials, indicating that they could be regarded as an alternative source of stem cells. [Keywords] placenta; adherent cells; stem cells [Abstract] Objective: In vitro isolation and culture of placental adherent cells in order to study its morphology, phenotype and differentiation characteristics. Methods: Type IV collagenase digested human placenta obtained mononuclear cells were inoculated in 100 mL / L FBS sugar DMEM medium, adherent after the routine exchange fluid passage, flow cytometry of their surface markers, drawing the growth curve. using β