Preparation of CdS quantum dots with L. monocytogenes coupling of antibodies.doc

 PAGE \* MERGEFORMAT 17 Preparation of CdS quantum dots with L. monocytogenes coupling of antibodies Of: Wang Ji Su Wan Yuanguo Jiangliu Ting Hsieh Ai-ling Cai Zhaoxia [Abstract] CdCl2 and Na2S as raw materials and mercaptoacetic acid as a stabilizer to the water phase synthesized and CdS quantum dots. The results show that the synthesis of CdS quantum dots the best conditions are: reaction time 3 h, [Cd2 +]: [ S2-] for the 2:1, 50 μL stabilizer, pH 8.0, reaction temperature 30 ℃. by EDC · HCl and NHS role, successfully L. monocytogenes IgG antibody coupled with CdS quantum dots. conjugates after The IgG CdS fluorescence intensity increased, the coupling of 4 times before. agglutination reaction and direct immunofluorescence experiments show that coupling of CdS quantum dots L. monocytogenes did not change the characteristics of antibodies in the fluorescence microscope can be quickly sensitive detection of Listeria monocytogenes. The method specificity, stability and reproducibility and can be used for rapid detection of pathogens in food. [Keywords:] quantum dots; Listeria monocytogenes; coupling; fluorescence intensity 1 Introduction CdS quantum dots as Ⅱ Ⅵ, is a common core-shell quantum dots of the main components [1]. CdS quantum dots with good optical properties, synthesis of fluorescent emission of different colors of water-soluble CdS quantum dots for biological labeling and multicolor nuclear Synthesis of shell quantum dots is important. For the preparation of CdS quantum dots of more [2 5], and more use of chemical liquid method. This method has mild reaction conditions, easy to control, uniform composition can be obtained, the purity nano-materials with high benefits. Listeria monocytogenes bacteria (Listeria monocytogenes, referred to as L. monocytogenes) is a way for people and animals suffering from meningitis, septicemia, and abortion, pathogens and other diseases, the mortality rate is very high. Detection of Listeria monocyto