Preparation of HBa recipe Liposome Research.doc

 PAGE \* MERGEFORMAT 16 Preparation of HBa recipe Liposome Research Author: Liu Juan, Hu Shui-Gen, Wang Guohua, Ke Qian Kun, Cai Ding-guo 【Abstract】 Objective To optimize the HB-Ⅰ a recipe liposomes improve the encapsulation efficiency. Methods Preparation of reverse-phase evaporation HB-Ⅰ a liposome, with encapsulation rate as an index, through the orthogonal experiment was optimized to process a prescription study, using HPLC determination of its encapsulation efficiency. Scanning transmission electron microscopy to observe the shape and appearance of the average particle size measured. Results The optimum ratio of lecithin prescription: cholesterol: HB-Ⅰ a = 400:200:60, the average encapsulation efficiency was (85.92 ± 0.61)%, average particle size is 11.25μm. Conclusion optimized prescription process is reasonable, encapsulation efficiency significantly increased. Keywords: HB Ⅰ a; Liposome; orthogonal experiment; entrapment efficiency AbstractObjectiveTo optimize the preparation and formulation of HB-Ⅰ a liposome and improve the entrapment efficiency. MethodsHB-Ⅰ a Liposomes were prepared by reversed-phase evaporation. Orthogonal design was adopted to screen the formulation of HB-Ⅰ a liposomes on the basis of the entrapment efficiency. The entapment efficiency of HB-Ⅰ a was determined by HPLC. The morphology and particle size of the preparation were examined by transmission electron microscope. ResultsThe optimum formula was PC: CH: HB-Ⅰ a = 400:200:60. The average encapsulation efficiency was (85.92 ± 0.61)%. The average diameter was 11.25μm.ConclusionThe formulation of HB-Ⅰ a is reasonable and can improve the entrapment efficiency. Keywords:HB  Ⅰ a; Liposome; Orthogonal design; Entrapment efficiency HB is a circular from the sea Limnology class molluscs Anodonta mussels Anodonta woodiana (Lea) extracted from a group of anti-tumor activity of glycoproteins substances. The activity of glycoproteins on the seve