Prunella vulgaris HPLC fingerprint of.docVIP

 PAGE \* MERGEFORMAT 16 Prunella vulgaris HPLC fingerprint of Author: Yang Hui, Zhao Yong Li Jun Guo Weiying 【Abstract】 Objective: To establish the fingerprint analysis of Prunella vulgaris, and different areas of the Prunella vulgaris comparative study of fingerprint patterns. Methods: oleanolic acid as a reference, using high performance liquid chromatography column was Hypersil C18, mobile phase was methanol: water: acetic acid (86:14:0.1) and the detection wavelength of 208nm, flow rate 1ml / min. Results: A total of 10 major peaks are marked, methodological study indicate that this study the establishment of analytical methods have better reproducibility and comparison of different origin with standard medicinal herbs Prunella fingerprint similarity. Conclusion: The method is stable, reliable, simple, in order to improve the quality control of herbs provide the basis for Prunella. Keywords: Prunella vulgaris; high-performance liquid chromatography; fingerprint To Labiatae Prunella vulgaris Prunella vulgaris Prunella vulgaris L. Plant dry ear, around the middle of China, the main production in Jiangsu, Zhejiang, Anhui, Henan and other places [1]. Prunella vulgaris wide range of clinical medicine, contains a “clear fire, eyesight, Sanjie, swelling” [2] the efficacy of modern research shows that Prunella vulgaris also has a lowering blood pressure, lowering blood sugar, immune suppression and anti-tumor effects, etc. [ 3]. At present, the market Prunella origin and more variable quality, lack of uniform quality and testing standards, usually measured one of the two compounds content approach to quality assessment, not fully reflect the overall quality of medicines. In this study, high-performance liquid chromatography fingerprint of Prunella vulgaris was the research, the results show that this method is stable, reliable and good reproducibility for quality control of medicinal herbs Prunella to provide an effective frame of refere