Radix verbena verbena Determination of glycosides.doc

 PAGE \* MERGEFORMAT 7 Radix verbena verbena Determination of glycosides [Abstract] Objective of the active ingredients for medicinal Verbena content standard. Method using high performance liquid chromatography (HPLC) method in medicine to determine the content verbena glycosides. Results verbena Verbena Radix glucosinolate content of not less than 0.30% . recovery was 100.96%, RSD 2.31%. Conclusion HPLC method is specific, accurate, reproducible, and can be used for quality control of medicinal herbs verbena. [Keywords:] Verbena; verbena glycosides; high performance liquid chromatography Verbenaceae Verbena plant is Verbena Verbena officinalis L. aerial parts dry, sexual bitter, cold, the liver, spleen; with blood stasis, cut malaria, detoxification benefits of water swelling and other effects [1]. Whip asiatica is one of its active compounds, in relatively high concentration of Radix, has anti-inflammatory, antibacterial, and enhance the role of immunity [2 3]. effectively detect the verbena verbena herbs to ensure the drug for clinical glycosides efficacy of positive significance. Therefore, this experiment its content. reported below. An instrument and reagent HPLC: Shimadzu LC-2010CTH, LC-Solution workstation. HPLC pure methanol and water as pure water, and other reagents were of alcohol. Reference substance: Verbena monophosphate (U.S. sigma companies, lot: 1077090) 2 Methods and Results 2.1 Chromatographic conditions Column: Dima C18 column (250 mm 4. 6 mm, 5 m); mobile phase: methanol - water (30:70); flow rate: 1.0 ml min-1, detection wavelength: 240; column Temperature: room temperature (25 ); number of theoretical plates verbena glycoside peak should not be less than 4000; chromatography shown in Figure 1. a reference substance b verbena verbena Verbena glycoside herbs 1 Figure 1 Figure verbena herbs HPLC 2.2 Preparation of control solution Weigh verbena glycosides precision reference substance amount, plus dissolv