Recombinant nematode anticoagulant protein rAcaNAP9 observed in rats and the effect of thrombus.docVIP

 PAGE \* MERGEFORMAT 9 Recombinant nematode anticoagulant protein rAcaNAP9 observed in rats and the effect of thrombus Study: Hong Tao, Liu Dong, Liu Yun Bao, Yin Wan, training with the text, Liu, Peng Fei Li [Abstract] Objective To observe the anti-recombinant nematode anticoagulant protein rAcaNAP9 carotid artery thrombosis in rats. Methods 40 SD rats were randomly divided into rAcaNAP9 low dose group (40 g / kg), medium dose group (200 g / kg ), high dose group (1 mg / kg), positive control group (heparin, 1650 U / kg) and model control group were 5 groups of 8. isolated rat common carotid artery on one side, from the mouse tail After intravenous administration, the thrombus formation apparatus with constant electrical stimulation of the arteries 5min, detection of blockage of blood vessels by stimulating the degree. Results of model control group, the degree of vascular congestion and rAcaNAP9 low, middle and high difference between the 3 dose groups were statistically significant (P lt;0.05 or 0.01). rAcaNAP9 recombinant nematode anticoagulant protein with anti-rat carotid artery thrombosis results. [Keywords:] Recombinant nematode anticoagulant protein; rAcaNAP9; anti-thrombosis; rat Abstract: Objective To investigate the antithrombotic role of recombinant nematode anticoagulant protein rAcaNAP9 in rat’s common carotid artery (CCA). Methods Forty rats were randomly divided into model control group, positive drug group (heparin, 1650 U / kg), and low (40 g / kg), middle (200 g / kg) and high dose (1 mg / kg) rAcaNAP9 groups. After isolation of unilateral CCA and intravenous injection of various drugs through caudal vein, the CCA was stimulated by a constant current for 5 minutes and its blood flow volume was recorded. Results The blood flow volume in 3 rAcaNAP9 groups was superior to that in model control group (P lt;0.01 or 0.05). Conclusion The rAcaNAP9 has the antithrombotic effect on rat’s CCA. Keywords:: recombinant nematode anticoagula