Recombinant Psilencer1.0U6siRNAstat3 plasmid cultured rat aortic smooth muscle cells.docVIP

 PAGE \* MERGEFORMAT 9 Recombinant Psilencer1.0U6siRNAstat3 plasmid cultured rat aortic smooth muscle cells [Abstract] Objective To observe Psilencer1.0 U6 siRNA stat3 recombinant plasmid on rat aortic smooth muscle cells (RSMCs) anti-proliferation mechanism. Methods The method of tissue culture RSMCs, application of liposome method to re-Psilencer1.0 U6 siRNA stat3 plasmid in vitro RSMCs, using phase contrast microscopy, MTT colorimetric proliferation observed in vitro RSMCs. Results After 72 h culture, a difference can be seen under the microscope plasmid significantly reduced cell proliferation, MTT test shows the recombinant plasmid group was significantly higher inhibition rate RSMCs. Psilencer1.0 U6 siRNA stat3 concluded that the recombinant plasmid can inhibit RSMCs proliferation. [Keywords:] aortic smooth muscle cells, STAT3, recombinant plasmid Psilencer1.0 U6 siRNA stat3 Vascular smooth muscle cells (VSMC) migration and proliferation of inner layers of vascular proliferative diseases in recent years the research focus and control difficult (1.) Found that changes in smooth muscle cell phenotype (from contractile into a secretory makes smooth muscle cells acquire the ability of proliferation and migration, resulting in transplant or neointimal hyperplasia after injury, resulting in vascular restenosis (2). with a view of smooth muscle cell proliferation and metastasis by intracellular signaling pathway regulated (3,4). transcriptional signal transducers and activators pathway (STAT3 on behalf of a signal from the membrane to the nucleus conduction system, activation can selectively activate its substrates - STAT, and the corresponding activation of Cyclin D1, Bcl xL and C myc downstream target genes, regulation of cell proliferation, differentiation and apoptosis, promotion of VSMC proliferation may play an important role in the process (5). Psilencer1.0 U6 siRNA stat3 is created by RNA interference can inhibit the expression of recombinant