Rat bronchoalveolar lavage Improvement.docVIP

 PAGE \* MERGEFORMAT 6 Rat bronchoalveolar lavage Improvement Author: Zhou Yanan, HAN Jun-wei, Gao Jun-ling [Keywords:] in rat bronchial lavage Bronchoalveolar lavage (bronchoalveolar lavage, BAL) is to study the bronchial and lung diseases, one of the key technologies, especially in the application of more extensive animal experiments. As people continue to in-depth study of lung disease, BAL is not only technology for the treatment of diseases, but also by analyzing the fluid composition and content, as certain diseases or advanced stage when diagnosed early diagnosis of secondary indicators. However, due to the existing irrigation technologies are not standardized, making the indicator difficult to standardize. I experiment a large number of repetitive operation, to sum up some experience and attention to the matter, in the hope of the technology has been drawing on the normative operation. 1 Data and methods 1.1 Experimental Animal SPF-class male SD rats (purchased from Beijing Tong Lihua Victoria Animal Experimental Center), weighing 200 ~ 240g. 1.2 Experimental Methods intraperitoneal injection 10% chloral hydrate 10ml/kg weight, the rats were killed rapidly excessive anesthesia. Supine on the operating table fixed. Disinfection neck skin, along the ventral midline neck incision in the neck to do, after a throat of about 1cm long, blunt hemostat, after separation of subcutaneous connective tissue and muscles exposed the trachea. In the thyroid cartilage and the first cartilage ring or between the first and second cartilage ring with suture ligation, in the third, four or four or five cartilaginous rings to do a little between the ‘T’ shaped incision, the external diameter of 1.8 ~ 2.0mm of blunt infusion needle into the trachea tube cavity is about 2cm, and use suture fixation. Then there will be three-way pipe prior infusion tube connected to the needle the other side, everything is ready, the way to use drip of saline solution preheated (37 ℃ ), t