Quantitative image analysis of thin-layer chromatography Emodin in Polygonum multiflorum.docVIP

 PAGE \* MERGEFORMAT 10 Quantitative image analysis of thin-layer chromatography Emodin in Polygonum multiflorum Author: Tsai Lai Xiaohui Ge Hayakawa [Abstract] Objective To study the imaging method of thin-layer chromatography determination of Emodin in Polygonum multiflorum. Way to n-hexane - ethyl acetate - formic acid (15:3:0.3) as the agent to silica gel G as the stationary phase started Emodin standard samples, and fleece-flower root extracts, using thin-layer chromatography spots imaging system processing will be spot information converted into a quantitative spectral peak information. The results of emodin standard curve is in 0.2 ~ 1.0 μ g linear range, correlation coefficient r = 0.993. Emodin in Polygonum multiflorum sample content 0.184 mg / g, RSD 1.0%, recovery of 100.9%, RSD 3.3%. TLC imaging findings can be applied to the quality of fleece-flower root medicines management control, with the corresponding launch system can be extended to a number of applications. [Keywords:] Emodin Polygonum multiflorum imaging quantitative analysis of thin-layer chromatography Traditional Chinese medicine Polygonum multiflorum Thunb dry roots, with detoxification, elimination carbuncle, relax bowel functions, can be used to treat sore M. scrofulaceum epilepsy, rubella itching, constipation, dry bowel and hyperlipidemia. The main chemical constituents of Polygonum multiflorum: emodin (emodin), chrysophanol (chrysophanol), rhubarb (physcion), emodin 1,6 - dimethyl ether (emodin-1 ,6-dimethylether), emodin -- 8 - Ether (questin), ω -hydroxy-emodin (citreorosein), ω -hydroxy-emodin -8 - methyl ether (questinol), 2 - acetyl-emodin (2-acetylemodin), emodin-8-O - β -D-glucoside (emodin-8-O-β -D-glucoside), emodin-8-O-β -D-glucoside (Physcion-8-O-β -D-glucoside) and so on. One is a hydroxy anthraquinone emodin derivatives. Molecular formula C15H10O5. Orange needle-like crystals, melting point 256 ~ 257 ℃ ; do not dissolve in water, soluble in ethanol, slightly solubl