Qi Xiaoji side containing serum NKN28 pairs of human gastric cancer cells in vitro.docVIP

 PAGE \* MERGEFORMAT 11 Qi Xiaoji side containing serum NKN28 pairs of human gastric cancer cells in vitro [Keywords:] gastric cancer; serum; tumor; rat Consumer product Qi Fang (Yiqi Xiaoji Recipe, YQXJR) is based Ruijin Hospital, Shanghai Jiaotong University School of Medicine SHEN Xiao-heng, director of traditional Chinese medicine, Professor 10 years of clinical experience dedicated to cancer research and clinical practice with a new generation of sum out of anti-cancer compound Clinical observation of gastric cancer have better therapeutic effect. This experiment using Qi negatively side containing serum, in vitro incubation of human gastric cancer NKN  28 cells, by methyl thiazolyl tetrazolium (methyl thiazolyl tetrazolium, MTT) cell proliferation inhibition assay to study the side in vitro cultured human gastric cancer NKN  28 cells in vitro. 1 Materials and methods 1.1 The experimental material SD rats 50, male, clean-level animals, body weight (250 ± 30) g, from Ruijin Hospital, Shanghai Jiao Tong University School of Medicine animal rooms available; human gastric adenocarcinoma cell line NKN  28 cells, by Shanghai Ruijin Hospital Gastroenterology Laboratory incubated available; Yiqi negatively were mainly from Astragalus, Scorpio, and Hedyotis diffusa etc., raw drugs were purchased from Shanghai Ruijin hospital, pharmacy, pharmacy on behalf of the Chinese and fried, Shanghai Institute of Experimental medicine Room condensed, containing health dose of 2.8 g / ml; RPMI  1640 culture medium, trypsin, Gibco company for packaging and batch number 31800  022; newborn bovine serum, Hangzhou Institute of Chinese holly leaf bio-engineering material products; MTT, Shanghai Seedling Cell Biology Technology Co., Ltd.; DMSO (dimethyl sulphoxide, DMSO), the United States Fisher Scientific World’s Hong Kong office products; TE2000  U multi-inverted phase contrast microscope, Japan Nikon products; MK3 microplate reader, Finland Lei Bo products. 1.2