Puhuang high-performance liquid chromatographic fingerprint pattern recognition research.docVIP

 PAGE \* MERGEFORMAT 7 Puhuang high-performance liquid chromatographic fingerprint pattern recognition research Author: XU Xiu-ying Cheng Yi-min FU Shan-quan ZHANG Zeng-tao [Abstract] Objective To establish the determination method Puhuang fingerprint. Methods RP-HPLC method to measure Puhuang fingerprints, chromatographic conditions: column for the Shimadzu C18 (150 mm * 4.6 mm, 5 μ m); mobile phase was methanol - water (54:46); flow rate of 1.0 ml * min-1; detection wavelength 254 nm; column temperature was 25 ℃ . Resulted in the creation Puhuang fingerprint standard. Conclusion The fingerprinting method is simple, rapid and reproducible, can be used as a quality control indicator Puhuang. [Keywords:] Puhuang fingerprint fuzzy pattern recognition Abstract: Objective To establish the method of fingerprint in Typha angustifolia L. by HPLC. MethodsThe fingerprint was determined by HPLC. The analysis was carried out on Shimadzu C18 column (150 mm * 4.6 mm, 5μ m). The mobile phase was MeOH-H2O (54:46) with a flowrate of 1.0 ml * min-1at 254 nm and the seperation was performed at 25 ℃ . ResultsThe standard of fingerprint in Typha angustifolia L. was established.ConclusionThe method is simple, quick and repeatable. It can be used in quality assessment of Typha angustifolia L.. Keywords:: Typha angustifolia L.; Fingerprint; Fuzzy pattern recognition Puhuang for Typhaceae Typha angustifolia Typha plants TyPha angustifolia L. Oriental Typha Typha orientalis Pres1 or the same plant dry pollen, with bleeding, Huayu, Tonglin the efficacy [1]. Commonly used in a variety of bleeding disorders and confidant of pain treatment. Modern pharmacological studies have shown that the water extraction and alcohol precipitation Puhuang liquid with a significant increase in coronary blood flow and reduce heart and brain oxygen consumption, dilation of blood vessels, reducing blood lipids and anti-atherosclerosis and many other activities. Determination of effective components