Quantitative pathology of severe pneumonia model.docVIP

 PAGE \* MERGEFORMAT 15 Quantitative pathology of severe pneumonia model Of: Chen Ye Min, Hou Tongchuan, Parkinson contend, Shen Hung [Abstract] Objective Quantitative stereological study SD (Sprague Dawley rats with severe bacterial pneumonia, three-dimensional model of the pathological changes of morphological characteristics and. Methods SD rats were randomly divided into model group, treatment group and control group (n = divided into 3 groups, each group 8 the first two groups, control group, each group 4). Among them, the model group and observation group were inoculated with different doses of the same concentration of Klebsiella pneumoniae bacterium, the control group and model group dose vaccination the same saline. were the first 2,4,6 days after inoculation, animals were sacrificed. specimens from lung tissues, HE staining, each slice were taken 3 to 4 fields of vision, with the Image-Pro image analysis software were red blood cell volume density test lung parenchyma, bronchial residual lumen volume density, alveolar volume density. Results With the inoculation time, the model group, the lung parenchyma observation group coefficient of variation of red blood cell volume density increased, especially in the model group increased Obviously, the model group, the residual lumen bronchial observation group coefficient of variation of volume density decreased, especially in model group was significantly, the model group and observation group coefficient of variation of volume density of alveoli became smaller, especially in the model group significantly. The control group no significant change. Conclusion With the inoculation time, the model group, more severe lesions were observed, especially in model group, the more apparent. Stereology in the pathology manifested as follows: red blood cells in the lung tissue volume increased, the residual bronchus lumen ratio of the original lumen volume decreased alveolar volume ratio and reduced the lung parenc