Rehabilitation medicine heart after acute myocardial infarction in rats bone marrow mesenchymal stem cells in protection.docVIP

 PAGE \* MERGEFORMAT 17 Rehabilitation medicine heart after acute myocardial infarction in rats bone marrow mesenchymal stem cells in protection Of: Li Guangbin, Sujin Ling, Jiangxi Juan, TRADITIONAL [Abstract] Objective To explore the heart of Chinese medicine rehabilitation after acute myocardial infarction in the rat bone marrow mesenchymal stem cells (BMSCs protective effect and its mechanism. The method has been combined density gradient centrifugation and cultured adherent screening method and purified BMSCs, with coronary artery ligation rat model of acute myocardial infarction, transplantation prior to the 5 - bromodeoxyuridine (Brdu marker of transplanted cells, cardiac rehabilitation patients fed by traditional Chinese medicine combined with stem cell transplantation. 2 weeks after transplantation, ELISA Method to Examine mouse serum vascular endothelial growth factor (VEGF content, animals were sacrificed and immunohistochemistry was used to detect areas of myocardial injury VEGF protein expression, Brdu positive transplanted cells and the number of CD34 + angiogenesis. Results cardiac rehabilitation combined with stem cell transplantation for rat myocardial infarction, can improve the survival rate of transplanted cells (with the cell transplantation group P lt;0.05, increased serum VEGF levels (with the cell transplantation group P lt;0.05, VEGF protein expression increased infarct area, infarct area to increase the number of new blood vessels. Conclusion heart rehabilitation medicine Kang rats transplanted stem cells after acute myocardial infarction has protective effects, which may be combined with the heart rehabilitation stem cell transplantation can be more effective in promoting angiogenesis related damage zone. [Keywords:] heart rehabilitation, myocardial infarction, bone marrow mesenchymal stem cells, angiogenesis Abstract: ObjectiveTo discuss the protective action on transplanted BMSCs by Chinese traditional medicine XinFuKang