Rehmannia glutinosa polysaccharide extraction and preliminary purification of_0.docVIP

 PAGE \* MERGEFORMAT 13 Rehmannia glutinosa polysaccharide extraction and preliminary purification of [Abstract] Objective Optimization of Rehmannia glutinosa polysaccharides optimal extraction and purification. Methods orthogonal experiment, the amount of water (A), extraction time (B), extraction times (C) as the three factors in order to receive dry paste rate (%) and polysaccharide content (%) as an indicator, the use of single factor experiments screen alcohol precipitation concentration and optimal concentration of calcium chloride de-protein. The results of various factors on polysaccharide content and rate of income paste composite score did not significantly influence, alcohol Journal of the optimal concentration of 80%, the best concentration of calcium chloride removal of protein (W / V) is 7%. Conclusion Rehmannia polysaccharide optimum extraction process A3B1C3, that is 8 times the amount of water, extracted 2 times 4 h, the concentration of alcohol precipitation is 80% off the optimal concentration of protein is 7%. [Keywords:] Rehmannia glutinosa polysaccharides; extraction process; alcohol precipitation concentration; removal of protein Plants from the Scrophulariaceae Rehmannia Rehmannia Rehmanniag lutinosa Libosch fresh or dried root, as commonly used traditional Chinese medicine. Huang is processed products to yellow, filled with pith essence, the efficacy of Yin blood for liver and kidney, head faint ringing in the ears , waist virtual knees, Gu Zheng, hot flashes, heat diabetes, blood deficiency chlorosis, palpitation, irregular menstruation, uterine bleeding in blood, dizziness, tinnitus, premature graying embolism. anxiolytic Rehmannia Rehmannia glutinosa polysaccharide is a major One of the active site [1]. In order to obtain the ideal extraction efficiency, we carried out Rehmannia glutinosa polysaccharide extraction, preliminary purification. are reported below. 1 apparatus and materials 1.1 Instrument UV-2201 UV-visible