Red copper protein compound water Jie Ji Mass spectra of the active site.doc

 PAGE \* MERGEFORMAT 12 Red copper protein compound water Jie Ji Mass spectra of the active site [Abstract]  compounds of copper metal enzyme mimics, by polyacrylamide gel electrophoresis study their effects on the hydrolysis of myoglobin, using high performance liquid chromatography electrospray ionization mass spectrometry measurement method instead of the traditional combination of N-terminal amino acid sequence analysis matrix-assisted laser desorption ionization time of flight mass spectrometry or electrospray ionization mass spectrometry to detect peptide fragments obtained by molecular weight, thus to determine the active site of hydrolysis. 10093.0,10388.0,6876.0 and the results of molecular weight of 6581.0 4 fragments, corresponding to in Gly1 91, Gly1 Ala94, Ser92 Gly153 and Thr95 Gly153, that Cu2 + with myoglobin His93 side chain coordination, hydrolytic cleavage at the before and after the first two along the peptide bond, namely: Gln91 Ser92 and Ala94 Thr95, hydrolysis reaction with high selectivity. in front of the active site His93 characteristics consistent with previous studies, while His93 along the active site, that is, His93 Ala94 Thr95 Thr95 N terminal sequence is a new site. [Keywords] copper, myoglobin, high performance liquid chromatography electrospray ionization mass spectrometry, hydrolysis 1 Introduction Hydrolysis of peptide and protein chemistry and biochemical processes are the most important reaction is one of biochemical analysis and protein structure and function of one of the most important tasks. Protein sequence analysis, protein structure and function, protein and nucleic acid interaction and synthesis of new chemical drugs, etc. are involved in such reactions. At present, can be used to promote hydrolysis of peptides and proteins by proteolytic enzymes and more, as they can hydrolyze phospholipids, proteins and DNA of these macromolecules. but the small number of enzymes, can not meet the protein structure a