Re-building species of black seed morphological markers and ISSR markers in genetic differentiation.doc

 PAGE \* MERGEFORMAT 17 Re-building species of black seed morphological markers and ISSR markers in genetic differentiation Of: Chen Zhao, Rui Ye Chao, Zhang Rui, Liwei Yang, Wang Li [Abstract] This paper re-building of the black seeds of two varieties within species Paris thibetica - black seed re-building of the original variant of Paris thibetica var. Thibetica black seed with no flap polyphylla Paris thibetica var. Apetala in the morphological and molecular markers on ISSR Differences were studied and compared. morphological characteristics analysis showed that the re-building of the original black seed varieties with free flap re-building black seed length and width of the sepals, anther number, filaments and long seven floral traits were significantly different, but by the principal component Analysis of the map can not clearly distinguish the two variants, two variants of the general morphological differentiation was not obvious. ISSR analysis, from 100 ISSR primers, 8 primers were screened, among the 300 ~ 1500 bp band were amplified with 36, One variant of the black seed weight characteristics of the original building with a 5, no re-building expansion valve characteristics of black seed with 8. [Keywords:] black seed variety of morphological differences re-building ISSR Abstract: The morphological differentiation analysis on Paris thibetica var. Thibetica and Paris thibetica var. Apetala was accomplished to indicate that the morphological differentiation between those two varieties were not significant, while the differentiations of the floral organs were significant. Generally, Paris thibetica var. thibetica and Paris thibetica var. apetala could not be classified by principal component analysis. In ISSR analysis, 8 proper primers were selected from 100 ISSR primers. And 36 bands were amplified by ISSR-PCR, of which length range was 300bp to 1500bp. Paris thibetica var. thibetica had 5 characteristic bands, while Paris thibetica var. apet