Qushi Huayu Decoction on fatty liver in rat adiponectin free fatty acid metabolism in the path of.doc

 PAGE \* MERGEFORMAT 13 Qushi Huayu Decoction on fatty liver in rat adiponectin free fatty acid metabolism in the path of Authors: Li Hongshan, FENG Qin, Xu Lili Chen Shaodong Xue-Mei, Hu Yang 【Abstract】 Objective: To investigate the prevention and treatment Qushi Huayu Decoction of fatty liver and its effect on adiponectin (adiponectin, ADP)  free fatty acids (free fatty acid, FFA) metabolism in the path effects. Methods: Wistar male rats to carbon tetrachloride (carbon tetrachloride, CCl4) subcutaneous injection of compound preparation of high-fat low-protein diet in rat models of fatty liver. In the 2 weeks after the beginning of modeling, the 40 model rats were randomly divided into model group and the high, medium and low-dose Qushi treated group, by gavage medication for 2 weeks. Detection of liver triglyceride (triglyceride, TG), FFA content; observation of pathological changes in liver tissue (hematoxylin and eosin stain, oil red O staining); enzyme-linked immunosorbent assay of serum and liver tissue of ADP Adiponectin receptor-2 (adiponectin receptor 2, AdipoR2), malonyl-coenzyme A (malonyl  coenzyme A, malonyl  CoA), adenosine monophosphate-activated protein kinase (AMP  activated protein kinase, AMPK), fatty acid synthase (fatty acid synthase, FAS), acetyl coenzyme A carboxylase (acetyl  coenzyme A carboxylase, ACCase), carnitine acyl transferase 1 (carnitine palmitoyltransferase  1, CPT  1) content changes. Results: Compared with the normal group, model group, liver tissue samples apparent macrovesicular fatty degeneration; liver tissue TG, FFA, FAS, ACCase, and malonyl  CoA content was significantly elevated serum ADP liver tissue AdipoR2, AMPK and CPT  1 levels decreased significantly. High, medium and low doses of Qushi Huayu Decoction can make fatty liver pathological changes in liver of rats significantly reduced and liver tissue TG, FFA content decreased; high doses may make the model Qushi Huayu rat liv