Retinoic acid on thyroid carcinoma cell line SW579 cell proliferation.doc

 PAGE \* MERGEFORMAT 10 Retinoic acid on thyroid carcinoma cell line SW579 cell proliferation [Abstract] Objective To observe the all-trans retinoic acid (all-trans-retinoic acid, ATRA) on thyroid carcinoma cell line SW579 induced differentiation in cell proliferation. Method to a final concentration of 10-7,5 10-7 ,10-6, 5 10-6,10-5 mol / L of ATRA effect SW579 cell lines were observed under the inverted microscope and morphological changes in cell growth, cell viability by MTT colorimetric assay, cell cycle by flow cytometry. Results of all-trans retinoic acid causes cells to a benign differentiation, inhibit tumor cell proliferation, the cells remain in S phase. Conclusion ATRA on thyroid carcinoma cell line SW579 in a dose dependent Inhibition of proliferation, and at low concentrations (10-7,5 10-7,10-6 mol / L could induce differentiation of cells. [Keywords:] all-trans retinoic acid of thyroid cancer cell proliferation and cell differentiation Abstract: Objective To observe the influences of all-trans-retinoic acid (ATRA) on proliferation of of thyroid squamous cell carcinoma cell line (SW579) in inducing differentiation in vitro.Methods Treated with different doses of ATRA (final concentration of 10-7 , 5 10-7,10-6,5 10-6,10-5 mol / L), the cells were observed with an inverted microscopea convert microscope in shape, The the proliferation of the cells was observed with MTT and the cell cycle was measured by flow cytometry. Results After cells were treated with ATRA, the malignant phenotypes of cells gradually changed to the mature cells. The proliferation index decreased markedly. The cell cycle was arrested at S stage.Conclusions ATRA can inhibit the proliferation of the cell line SW579 with a dose-dependent manner, and maybe the thyroid cancer cells were induced differentiation in low concentration (10-7mol / L, 5 10-7mol / L ,10-6mol / L. Keywords:: ATRA; thyroid squamous cell carcinoma; proliferation; differentiation Induced tum