RNAi silencing of VEGF expression in colon cancer liver metastasis.doc

RNAi silencing of VEGF expression in colon cancer liver metastasis Of: Wang Chongjiang, Fan Yu, Xu, the Pang Liqun [Abstract] Objective: To study the vascular endothelial growth factor (vascular endothelial growth factor, VEGF on colorectal cancer liver metastases. Methods: According to the VEGF gene mRNA synthesized small interfering RNA, using fluorescent real-time quantitative PCR detection of VEGF mRNA levels, respectively, soft agar culture test and Boyden model to investigate cell anchorage independent growth and invasiveness of cancer cells. to splenectomy model of colorectal cancer liver metastases were established, a different approach to vaccination model of cancer cells was observed on colorectal cancer liver metastases effect. Results: VEGF gene transfection group mRNA levels were significantly lower in a dose and time dependent. by VEGF siRNA transfected cells treated the number of soft agar colony formation and significantly reduced the number of penetrating cells in a dose-dependent (P lt;0.05, P lt;0.05. the body was found that the control group, all 10 liver metastases of colorectal cancer occurred, but only one appeared siRNA liver metastasis group, the difference was significant (P lt;0.01. Conclusions: VEGF gene liver metastasis in colorectal cancer plays an important role in RNAi technology to silence the expression of VEGF gene, can inhibit liver metastasis of colorectal cancer cells. [Keywords:] intestinal cancer, liver metastasis, vascular endothelial growth factor, RNA interference [Abstract] Objective: To study the effects of vascular endothelial growth factor (VEGFon liver metastasis of colon cancer. Methods: After human colon cancer cell line LS174T were transfected by VEGF small interfering RNA (siRNA, the mRNA level was determined by real time RT PCR.The anchorage independent growth was examined using clone formation in soft agar, and invasion ability was evaluated by boyden chamber model. Thirty nude mice model of colorecta