RNAi silencing of VEGF expression in colon cancer liver metastasis.docVIP

RNAi silencing of VEGF expression in colon cancer liver metastasis Of: Wang Chongjiang, Fan Yu, Xu, the Pang Liqun [Abstract] Objective: To study the vascular endothelial growth factor (vascular endothelial growth factor, VEGF on colorectal cancer liver metastases. Methods: According to the VEGF gene mRNA synthesized small interfering RNA, using fluorescent real-time quantitative PCR detection of VEGF mRNA levels, respectively, soft agar culture test and Boyden model to investigate cell anchorage independent growth and invasiveness of cancer cells. to splenectomy model of colorectal canc