RNAi silencing survivin gene in pancreatic cancer cell proliferation apoptosis and cell cycle of.docVIP

 PAGE \* MERGEFORMAT 12 RNAi silencing survivin gene in pancreatic cancer cell proliferation apoptosis and cell cycle of Of: Tian Weijun Jin-Wei Zhao Song Xiaobin Abstract Objective RNAi silencing survivin gene in pancreatic cancer cell lines on tumor cell apoptosis, cell cycle and proliferation changes. Methods targeted survivin in pancreatic cancer cells transfected with RNAi vector, Western blot detection of gene silencing effect, MTT method and flow cytometry to detect cell proliferation, apoptosis and cell cycle. Results survivin gene silencing efficiency of up to 85%; MTT assay showed that survivin silencing interference group and the non-specific cell proliferation compared with vector control group was significantly higher (P lt;0.01). Flow cytometry showed that apoptosis in the interference group was significantly higher than non-specific plasmid control group (P lt;0.01); G2 / M phase was significantly higher than non-specific plasmid control group (P lt;0.01 or P lt;0.05), while the S period was significantly lower (P lt;0.05). Conclusion survivin gene silencing can cause pancreatic cancer cell cycle arrest and promote apoptosis, inhibit cell proliferation. Keywords: RNAi; survivin; apoptosis; cell cycle; pancreatic cancer Pancreatic cancer development and invasion, metastasis is a multi-gene participation, multi-step process occurs, in which survivin (survivin) are a class of pancreatic cancer, play a very important role in the development of cancer gene 1, its a variety of important biological processes involved in the cell, including transcriptional regulation, embryogenesis, cell differentiation, tumor formation and metastasis 1,2. the expression of survivin in embryonic rich, but the expression in mature tissues is minimal or absent, but in pathological conditions, including pancreatic cancer, many malignant tumors were found to have high levels of survivin expression, and its expression and malignant level and was positively co