Rosiglitazone delay the development of vascular calcification in diabetic rats.doc

 PAGE \* MERGEFORMAT 16 Rosiglitazone delay the development of vascular calcification in diabetic rats [Abstract] Objective: To observe the rosiglitazone on the treatment of vascular calcification in diabetic rats and its effects on bone transcription factor Msx2 expression. Methods: high fat diet plus low-dose intraperitoneal injection of streptozotocin treatment on the basis of plus vitamin D3 and nicotine treatment of diabetes rat model with vascular calcification (vascular calcification in diabetic group), to give some vascular calcification in diabetic rats fed rosiglitazone (2 mg kg-1, day 1 for 8 weeks) treatment (rosiglitazone group), while establishing the control group. 21 week experiment, rats were sacrificed each experimental group to detect the metabolism of rats, the blood vessels von Kossa staining, detection of blood vessels calcium content and alkaline phosphatase activity, application of real time PCR method for detection of Msx2 mRNA and protein was detected by Western blot Msx2 protein expression in the blood vessels. Results: Compared with the control group, diabetic rats with vascular calcification in vascular memory diffuse calcium deposition, elevated blood calcium levels, alkaline phosphatase activity increased, Msx2 mRNA and protein expression was significantly increased (all P lt;0.05); and after rosiglitazone treatment, the deposition of calcium in the blood vessels significantly reduced, calcium content and alkaline phosphatase activity decreased by 8.37%, 10.59%, Msx2 mRNA and protein expression was also decreased by 36.73%, 42.55% (with diabetes compared with vascular calcification, both P lt;0.05). Conclusion: Rosiglitazone may delay the development of vascular calcification in diabetic rats, and calcification of blood vessels can reduce the expression of Msx2. [Keywords:] Type 2 diabetes; vascular calcification; transcription factor Msx2; rosiglitazone; rat Compared with non-diabetic patients, type 2 diabetic patients