Rosuvastatin on neonatal rat cardiac fibroblast proliferation and apoptosis.doc

 PAGE \* MERGEFORMAT 13 Rosuvastatin on neonatal rat cardiac fibroblast proliferation and apoptosis OF: Ms Wong Wei Zhang Hailin Cui Ya [Abstract] Objective rosuvastatin (Ros) on the SD neonatal rat cardiac fibroblasts (CFs proliferation to explore the mechanism of apoptosis induced by CFs. Methods newborn SD rat CFs cultured in vitro, given alone 10-5,10-6,10-7,10-8 mol / L Ros, or with 10-5mol / L farnesoid pyrophosphate (FPP joint intervention, application of colorimetric MTT, BrdU colorimetric, Wright’s Giemsa staining inverted biological microscope, Hochest 33258 staining by fluorescence microscopy, flow cytometry technology, Ros observed the proliferation of CFs and its effect on apoptosis induced by CFs. Results Ros not only inhibit the proliferation of newborn SD rats and DNA synthesis of CFs and CFs induced apoptosis .10-5mol / L FPP partially reversed this effect. Conclusion Ros is the heart of effective anti-fibrosis drugs can not only inhibit fibrosis, but also reversing the fibrosis has occurred. [Keywords:] rat, fibroblasts, cell proliferation, apoptosis, rosuvastatin, pyrophosphate farnesoid Statins safe and reliable for its lipid-lowering effects and cardiovascular protection, widely used in clinical practice. However, the application of statins are mainly restricted to the scope of hyperlipidemia and coronary heart disease patients, for heart failure patients pros and cons of whether to apply and has been controversial. In this paper, a new lipid-lowering drugs observed rosuvastatin (Ros on SD neonatal rat cardiac fibroblasts (CFs proliferation inhibition and apoptosis induced by CFs for statins drugs in heart failure patients with reference to the feasibility. 1 Materials and methods 1.1 Apparatus and reagents CO2 incubator (Heal Force, biological safety cabinets (Heal Force, inverted biological microscope (Olympus 1 71, the camera (Canon DS126181, inverted fluorescence microscope (Nikon Ellipse TE2000 S, microplate reade