RP-HPLC determination of the more particles in the intestinal contents of ginsenoside Rg1.doc

 PAGE \* MERGEFORMAT 7 RP-HPLC determination of the more particles in the intestinal contents of ginsenoside Rg1 [Abstract] Objective To establish a more particles in the intestinal contents of ginsenoside Rg1 determination. Methods HPLC, column: C18 (4.6 mm × 250 mm, 5 μm), mobile phase: acetonitrile - water (80 : 20), flow rate: 1.0 mL / min, detection wavelength was 210 nm. the results of more particles in the intestine of ginsenoside Rg1 in the linear range of 0.125 ~ 4 μg, r = 0.999 7, the average recovery was 97.30%, RSD = 1.31 %. Conclusion The method is simple, reliable, reproducible, improves the quality control of intestinal more particles. [Keywords:] gut the more particles, ginsenoside Rg1, HPLC, determination Abstract: Objective To set up a method for the determination of ginsenoside Rg1 in Changyu Granules. Methods The content of ginsenoside Rg1 was determined by RP-HPLC on C18 column (4.6 mm × 250 mm, 5 μm). The mobile phase consisted of ethane nitrile and water (80:20). The flow rate was 1.0 mL / min. The detection wavelength was at 210 nm. Results Ginsenoside Rg1 showed a good linear relationship at the range of 0.125 ~ 4 μg, r = 0.999 7. The average recovery rate was 97.30%, RSD = 1.31%. Conclusion The method is simple with good reproducibility, and can be used for the quality control of Changyu Granules. Keywords:: Changyu Granules, ginsenoside Rg1, RP-HPLC, content determination The more particles of intestinal agreement Jiangyin Hospital of prescription, by the ginseng, Atractylodes etc., has completed the spleen and stomach, the role of lung benefits, the clinical use of chronic colitis, allergic colitis, intestinal disorders and other diseases. In this experiment high performance liquid chromatography (RP-HPLC) method for the determination of ginsenoside Rg1 in the preparation of the content, as the indicator of the quality control method is simple and operable. An instrument and reagent 1.1 Instrument Shimadzu L