RPHPLC determination of decoction of nutmeg and change the content of Myristicin.doc

 PAGE \* MERGEFORMAT 6 RPHPLC determination of decoction of nutmeg and change the content of Myristicin [Abstract] [Objective] The determination of nutmeg Nutmeg in the ether in the boiling water following the medicine or law and changes in the amount of fried out. [Methods] RP HPLC method, Tigerkin C18 column (150mm 4.6mm, 5m), using methanol: 0.025mol / L phosphoric acid (70:30) as mobile phase, flow rate of 1.0ml min-1, wavelength 282nm, column temperature 25 . [results] myristicin in 0.4g ~ 2.0g in the range of linear relationship between peak area Good r = 0.9998. [Conclusion] nutmeg in boiling water following the medicine or law, the nutmeg in the ether content in the decoction 2.45min peak, followed by decoction of nutmeg ether decreased gradually. [Keywords:] nutmeg; myristicin; RP-HPLC Abstract: [Objective] To measure the contents of Nutmeg ether, and its regularity varying with decoction time. [Method] RP HPLC method was used.The chromatographic conditions were listed below Tigerkin C18 column (150mm 4.6mm.5m), amobine phase composed of metranol-0.025mol / L phosphoric acid (70:30), a flow rate of 1mL min-1 and wave lengths was 282nm, column temperature was 25 . [Results] In the range of 0.4g ~ 2.0g, the linear was very well between the content of Nutmeg ether and peak area. [Conclusion] The contents of Nutmeg ether in decoction reached the peak at 2.45min after Nutmeg ether was added in boiling water. Keywords:: Nutmeg; Nutmeg ether; RP HPLC Nutmeg, meat plants for the meat nutmeg nutmeg myristica fragrans Houtt mature kernel [24]. Nutmeg Nutmeg ether is one of the main active ingredient, the detection method it has been many reports of domestic [5] In this paper, RP HPLC method at different time myristicin content in the decoction and changes in the law to determine the relevant Chinese medicine decoction of nutmeg best following the boiling time, and the curve to be considered by the ethers in 2.45min nutmeg When the highest cont