rRNA gene internal transcribed spacer region as a genetic marker of the application status and prospects of.doc

 PAGE \* MERGEFORMAT 18 rRNA gene internal transcribed spacer region as a genetic marker of the application status and prospects of [Abstract] With the continuous development of molecular biology techniques, rRNA gene internal transcribed spacer (ITS) as a genetic marker, has been widely used in classification and identification of microbial strains, identification and quality evaluation of Chinese herbal medicines, plant germplasm resource identification, animal phylogeny, genetic diversity and phylogenetic relationships, pathogen diagnostics, phylogenetic studies on different aspects of research. In recent years, the article on the rRNA gene internal transcribed spacer region of the application status and prospects were reviewed. [Keywords:] rRNA gene internal transcribed spacer region of genetic markers Genetic markers is traceable chromosomes, chromosome segments or to a particular locus in the transmission line at home in any kind of genetic traits, with heritability and identification of [1]. Any difference between biological phenotype of the gene mutation can serve as genetic markers. rRNA gene, also known as rDNA, which is encoded rRNA (ribosomal RNA) genes, the interval from the rDNA and the adjacent district. In eukaryotes, rRNA gene internal transcribed rDNA spacer region is on a non-coding region, which includes the ITS-1 (located between the 18SrDNA and 5.8SrDNA) and ITS-2 (located between 5.8SrDNA and 28SrDNA) region. In the region less affected by the external environment, with the coding region has evolved faster than the characteristics of [2,3]. The prokaryotic ITS sequence is 16S-23SrDNA internal transcribed spacer sequences, which are prevalent in prokaryotes, in the relatively stable environmental conditions change [3]. Higher plants in the rDNA is highly repetitive sequence units in tandem, and its ITS sequence is 18SrDNA-5.8SrDNA-26SrDNA spacer sequence, suffered a small selection pressure, evolution is faster in the species showed a h