RNA interference and nasopharyngeal carcinoma research.docVIP

 PAGE \* MERGEFORMAT 29 RNA interference and nasopharyngeal carcinoma research Abstract RNA interference (RNAi) is the use of double-stranded small RNA fragments efficiently and selectively degrade intracellular homologous mRNA, blocking gene expression in vivo, so that cells have the phenotype of target gene deletion [1]. In recent years, RNA interference studies at home and abroad to carry out quickly, and extended to many aspects, such as inhibiting the virus study, inhibition of multidrug resistance gene expression in the study of human parasites, gene therapy, cancer research. The pathogenesis of nasopharyngeal carcinoma with a number of pathogenic factors, experimental studies have shown that RNAi can be affected by a variety of ways to the growth of nasopharyngeal carcinoma cells, Therefore, RNAi may provide a new method of treatment of nasopharyngeal carcinoma. Keywords: RNA interference with small interfering RNA Nasopharyngeal cancer 1 RNAi mechanism of the basic processes and RNA interference (RNA interference, RNAi) by double-stranded RNA (double stranded RNA, dsRNA)-mediated specificity of the corresponding sequence of degraded mRNA, resulting in post-transcriptional gene silencing (posttranscriptional gene silencing, PTGS) phenomenon, with efficiency and a high degree of specificity, has become the study of gene function in a fast, simple and new method. At present there are three kinds of mechanisms of the main forms, namely, the level of transcription, translation level and post-transcriptional level. In which posttranscriptional level of RNAi in a variety of organisms to achieve the main way. 1.1 The transcription level of the interference mechanism of target genes by altering chromatin structure, so that gene transcription is restricted, closed expression system. dsRNA is degraded into a 21  23 nucleotides long RNA of small fragments, these small RNA molecules in the nucleus can induce histone H3 gene