The use of siRNA inhibited HER 2 expression in.docVIP

 PAGE \* MERGEFORMAT 16 The use of siRNA inhibited HER 2 expression in 【Key Words】 siRNA The Inhibition of HER  2 Expression by siRNA Abstract: Objective To inhibit the expression of HER  2 gene in MCF  7 human breast carcinoma cells by siRNA. Methods Five siRNAs targeting HER  2 were designed, and their corresponding expression vectors were constructed and transformed into MCF  7 cells. After selection , the stable transfected cells were obtained. The HER  2 mRNA abundance in the stable transfected cells was analyzed by RT  PCR. The HER  2 protein expression in these cells was analyzed by flow cytometry (FCM). The stable transfected cells were transplanted in nude mice, and the HER  2 protein expression in transplant tumor was detected with immunohistochemical method. Results siRNA 3P could effectively decrease the HER  2 mRNA expression in MCF  7 cells. The FCM data showed that the HER  2 protein expression in siRNA 2P and 3P transfected MCF  7 cells decreased. All the five siRNAs targeting HER  2 could decrease the tumorigenesis of MCF  7 cells in nude mice. Correspondingly, the HER  2 protein expression in transplant tumors was also reduced. Conclusion The siRNAs targeting HER  2 mRNA could effectively inhibit HER  2 expression in vivo and in vitro, and will be useful for gene therapy of breast cancer. Keywords:: HER  2; Small interfering RNA (siRNA); Breast cancer Abstract: The purpose of the use of siRNA inhibition in human breast cancer MCF  7 cells HER  2 gene expression. Methods for the HER  2 mRNA has designed five siRNA, and build the corresponding expression plasmid, the plasmid transfected MCF  7 breast cancer cells, stable expression strains screened using RT  PCR analysis of siRNA on the cell HER  2 mRNA Degradation; the use of flow cytometry analysis of cell-surface protein expression of HER  2; the use of nude mice in vivo tumorigenicity experiment, using immunohistochemistry to detec