Under hypoxic conditions HIF-1α in rat retina and the expression of P53 and correlation analysis.docVIP

 PAGE \* MERGEFORMAT 19 Under hypoxic conditions HIF-1α in rat retina and the expression of P53 and correlation analysis Authors: ZHANG Wei, LI Ruo-Xi, Xu Jianhua, Liu Zheli 【Abstract】 Objective: To investigate the hypoxic conditions, HIF-1α and the expression of P53 in the rat retina and their correlation. Methods: 56 rats were randomly divided into two groups, 28 in each group were housed in normoxia and 50mL / L oxygen warehouse. In feeding after 0,2,6,8,12,24, 48h were sacrificed 4, respectively, by light microscopy, electron microscopy, immunohistochemistry (SABC), and P53 measured HIF-1α expression and the associated analysis. Results: HIF-1α in hypoxia-2h post-began to express, 8h peak, 12 ~ 24h continuing strong expression, 48h expression decreased significantly. P53 at 2h after hypoxia began to express increased gradually after the expression, 24h after the peak expression, 48h expression decreased significantly. HIF-1α and the expression of P53 in the rat retina was significantly correlated (R = 0.902495). Electron microscopy results showed that hypoxia in the retina 6h began to change in retinal ultrastructure, 24h apoptosis reached a peak. Conclusion: The hypoxic conditions, HIF-1α, and P53 were expressed in the rat retina, with the expression of HIF-1α expression of P53 after reaching a peak reached a peak, both positively correlated. P53 expression reached a peak when the electron microscope results showed that apoptosis reached a peak. Keywords: hypoxia-inducible factor-1α P53 retinal hypoxia in the rat 0 Introduction Hypoxia causes cells to changes in physiological functions, resulting in body tissues, organs, loss of irrigation and damage. Hypoxia-inducible factor-1 (hypoxia inducible factor 1, HIF-1) was established in 1992 by Semenza, etc. found. HIF-1 can control erythropoietin (EPO), vascular endothelial growth factor (VEGF), glycolytic enzymes and a variety of gene expression. The most prominent feature of