TNF Leptin-Rb NF-B and PPAR- in alcoholic liver disease in rat liver cells.docVIP

 PAGE \* MERGEFORMAT 20 TNF Leptin-Rb NF-B and PPAR- in alcoholic liver disease in rat liver cells [Abstract] Objective To investigate the TNF @, NF-B, Leptin-Rb and PPAR- such as alcoholic liver disease in rats with liver inflammation, necrosis and fibrosis. Methods established by long-term gastrostomy in alcoholic rats liver disease were at 4,8,12,24 and 52 weeks by immunohistochemistry, in situ hybridization and RT-PCR to detect different stages of liver tissue TNF @, NF-B, PPAR- and Leptin- The expression of Rb, etc.. Results TNF @, NF-B and Leptin-Rb levels in the model group, liver cells, gradually increased the expression of the first 8 weeks, 24 weeks from the high level of expression continued until 52 weeks (P lt;0.01) . PPAR- in the control group and 4,8 in the model the strongest expression in liver cells, the expression of 12 weeks began to weaken. TNF @, and NF-B expression in hepatocytes was positively correlated with PPAR- is a negative correlation (P lt;0.01). Conclusions TNF @, and NF-B and the severity of liver cells are in good agreement relationship, Leptin-Rb expression in liver cells is consistent with the development of hepatic fibrosis, PPAR- is just the opposite. [Keywords:] of cytokines in rats with alcoholic liver disease ABSTRACT: Objective To investigate the expressions of TNF @, NF-B, leptin-Rb and PPAR- mRNA in liver tissues of rats with alcoholic fatty liver to identify the relationship between these factors and liver inflammation, necrosis and fibrosis. Methods Long- time rat model groups (gastric cannulation ethanol infusion models of ALD) were made. At the end of 4th, 8th, 12th, 24th and 52th week, the expressions of TNF @, NF-B and PPAR- protein and mRNA in rat liver tissues were observed with immunohistochemical method and in situ hybridization. Reverse transcription polymerase chain reaction was performed to determine the expression of Leptin-Rb mRNA. Results The expressions of TNF @, NF-B and leptin-Rb in rat a