不同生长期乌头根际土壤酶动态变化研究.PDFVIP

中草药 Chinese Traditional and Herbal Drugs 第 43 卷 第 7 期 2012 年 7 月 ? 1403 ? 不同生长期乌头根际土壤酶动态变化研究 吴清华，王光志*，马云桐，马 庆，徐瑞超 成都中医药大学药学院，中药资源系统研究与开发利用省部共建国家重点实验室培育基地，四川 成都 610075 摘 要：目的 研究不同生长期健康与患病乌头根际土壤酶活性的动态变化。方法 采用紫外分光光度法测定健康与患病乌 头不同生长期蔗糖酶、脲酶和磷酸酶的活性。结果 健康植株根际土壤蔗糖酶活性呈逐渐下降趋势，患病植株土壤蔗糖酶活 性呈逐渐增强的趋势；在相同生长期，健康植株土壤脲酶、磷酸酶活性均低于患病植株，脲酶在 3～5 月均呈逐渐增强的趋 势，酸性磷酸酶、碱性磷酸酶活性呈先降低后增强的趋势。结论 健康与患病乌头根际土壤酶活性差异较大，为乌头病害的 进一步研究提供了科学依据。 关键词：乌头；根际土壤；蔗糖酶；脲酶；磷酸酶 中图分类号：R282.13 文献标志码：A 文章编号：0253 - 2670(2012)07 - 1403 - 04 Dynamic changes of enzyme in rhizosphere soil of Aconitum carmichaeli at various growing stages WU Qing-hua, WANG Guang-zhi, MA Yun-tong, MA Qing, XU Rui-chao State Key Laboratory Breeding Base of Research and Development of Chinese Medicinal Resources, School of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu 610075, China Abstract: Objective To investigate the rhizosphere soil enzyme activities of healthy and diseased Aconitum carmichaeli at various growing stages. Methods The activities of sucrase, urease, and phosphatase were measured by UV spectrophotometry. Results The sucrase activity in rhizosphere soil of healthy plants showed a gradual decline trend, while a gradual increase in rhizosphere soil of diseased plant was observed; At the same growing stage, the urease and phosphatase activities in rhizosphere soil of healthy plant were lower than those of the diseased plants. The activity of urease showed a gradual increase from March to May and the activity of acid and alkaline phosphatases first decreased and then increased. Conclusion Rhizosphere soil enzyme activities of the healthy and diseased A. carmichaeli are quite different. The results may provide the scientific reference for disease study of A. carmichaeli. Key words: Aconitum carmichaeli Debx.; rhi