基于HPLC与主成分分析法区别西南地区不同产地花椒的地域差异 标记化合物的测定和鉴定.pdfVIP

现代食品科技 Modern Food Science and Technology 2015, Vol.31, No.8 Geographical Origin Discrimination of Zanthoxylum bungeanum from Southwest China Using HPLC and PCA: Identification and Determination of Marker Compounds 1 1 1 1 1 1 2 CHEN Huai-xuan , BAI Jin-rong , QIU Chen , ZHONG Kai , ZHAO Zhi-feng , GAO Hong , HUANGYi-na ( 1.College of Light Industry, Textile and Food Engineering, Sichuan University, Chengdu 610065, China) (2.Department of Public Health, West China Medical School, Sichuan University, Chengdu 610041, China) Abstract: The geographical origin of 15 samples of Zanthoxylum bungeanum fruits obtained from five different regions in Southwest China was differentiated. The pericarps of Z. bungeanum were extracted using methanol. Fractions were obtained by column chromatography and analyzed by high-performance liquid chromatography (HPLC) analysis. Principal component analysis (PCA) and partial least -square discriminant analysis (PLS-DA) were performed to develop classification models to differentiate between samples from Hanyuan and those from other regions of Southwest China. The results showed that samples from the Hanyuan region were significantly different from those obtained from the other regions; PLS-DA analysis showed a greater discriminatory effect than did PCA approach. In addition, the chemical compounds with variable influence on the projection (VIP) 1 from PLS-DA models and p 0.05 for unpaired Student’s t-test were considered relevant for group discrimination. These included neochlorogenic acid, chlorogenic acid, quercitrin, hydroxy -β-sanshool, and hydroxy -γ-sanshool, which could be potential markers to distinguish Hanyuan Z. b