Curcuma Longa Staining Effect on Histomorphology of the Testis.docVIP

Basic Science Macedonian Journal of Medical Sciences. 2012 Mar 15; 5(1):26-29. /10.3889/MJMS.1857-5773.2011.0209 Basic Science OPEN?ACCESS Curcuma Longa: Staining Effect on Histomorphology of the Testis Rosemary B Bassey , Ademola A Oremosu , Abraham AA Osinubi 1 2 2 1 2 Department of Anatomy, Faculty of Basic Medical Sciences, University of Uyo, Akwa Ibom State, Nigeria; Department of Anatomy, College of medicine, University of Lagos, Lagos, Nigeria Abstract Citation:BasseyRB,OremosuAA,OsinubiAAA. Background: The use of non-allergic, non-toxic and eco-friendly natural dyes has become a matter of significantimportanceduetotheincreasedenvironmentalawarenessinordertoavoidsomehazardous synthetic dyes. Curcuma Longa: Staining Effect on HistomorphologyoftheTestes.MacedJMedSci. 2012Mar15;5(1):26-29./10.3889/ MJMS.1957-5773.2011.0209. Method: The ethanolic extract of Curcuma longa was diluted using 1% acetic acid in 70% ethanol to a concentration of 0.2 g/ml. It was used to stain histological sections of the testes for 15 minutes. Curcuma longawasalsousedasacounterstainforHaematoxylin.Phytochemicalconstituentswereinvestigated. Key words: Curcuma longa; Stain; Dye; Testes. Correspondence: Rosemary B. Bassey . Department of Anatomy, Faculty of Basic Medical Sciences, University of Uyo, Akwa Ibom State, Nigeria. Tel: 234-8064853304, 8098555224. E- mail: rosemary_bassey@ Results: The Curcuma longa dye distinctly stained the seminiferous epithelium and interstitium yellow. Curcuma longa provided a good counter stain for Haematoxylin, taking up