PlantTissueCultureMedia-HorticulturalSciences.pptVIP

Plant Tissue Culture Media What’s really important? Plant Tissue CultureWhy does it work? Plant cells – Dedifferentiate Plant cell division- Somatic cells are diploid Mitosis – Chromosomes duplicate and divide Meiosis – The process of forming sex cells, 2n splits and become 1n gametes Major Constituents Salt Mixtures Organic Substances Natural Complexes Inert Supportive Materials Growth Regulators Macro-nutrient saltsWhat the ? NH4NO3 Ammonium nitrate KNO3 Potassium nitrate CaCl2 -2 H2O Calcium chloride (Anhydrous) MgSO4 -7 H2O Magnesium sulfide (Epsom Salts) KH2PO4 Potassium hypophosphate FeNaEDTA Fe/Na ethylene-diamine-tetra acetate H3BO3 Boric Acid MnSO4 - 4 H2O Manganese sulfate ZnSO4 - 7 H2O Zinc sulfate KI Potassium iodide Na2MoO4 - 2 H2O Sodium molybdate CuSO4 - 5 H2O Cupric sulfate CoCl2 - H2O Cobaltous sulfide Macronutrient salts Nitrogen – Influences plant growth rate, essential in plant nucleic acids (DNA), proteins, chlorophyll, amino acids, and hormones. Phosphorus – Abundant in meristematic and fast growing tissue, essential in photosynthesis, respiration. Potassium – Necessary for cell division, meristematic tissue, helps in the pathways for carbohydrate, protein and chlorophyll synthesis. Macronutrient salts Calcium - Involved in formation of cell walls and root and leaf development. Participates in translocation of sugars, amino acids, and ties up oxalic acid (toxin). Iron - Involved in respiration , chlorophyll synthesis and photosynthesis. FeNaEDTA = sodium salt of EDTA sequesters iron, making it available to plants. Magnesium - Involved in photosynthetic and respiration systems. Active in uptake of phosphate and translocation of phosphate and starches. Micronutrient salts Sulfur - Involved in formation of nodules and chlorophyll synthesis, structural component of amino acids and enzymes. Manganese - Involved in regulation of enzymes and growth hormones. Assists in photosynthesis and respiration. Micronutri