Antimicrobial Resistance, Virulence Factors and Genetic Diversity of Escherichia coli Isolates from Household Water Supply in Dhaka, Bangladesh.docVIP

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Antimicrobial Resistance, Virulence Factors and Genetic Diversity of Escherichia coli Isolates from Household Water Supply in Dhaka, Bangladesh.doc

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Antimicrobial Resistance, Virulence Factors and Genetic Diversity of Escherichia coli Isolates from Household Water Supply in Dhaka, Bangladesh

AntimicrobialResistance,VirulenceFactorsandGenetic DiversityofEscherichiacoliIsolatesfromHousehold WaterSupplyinDhaka,Bangladesh PrabhatKumarTalukdar1,2,MizanurRahman1,MahdiaRahman1,AshikunNabi1,ZhahirulIslam1, M.MahfuzulHoque2,HubertP.Endtz1,3,MohammadAminulIslam1* 1FoodSafetyResearchGroup,CentreforFoodandWaterborneDiseases,InternationalCentreforDiarrhoealDiseaseResearch,Bangladesh(icddr,b),Mohakhali,Dhaka, Bangladesh, 2Department of Microbiology, University of Dhaka, Dhaka, Bangladesh, 3Department of Medical Microbiology and Infectious Diseases, Erasmus MC, UniversityMedicalCenterRotterdam,Rotterdam,TheNetherlands Abstract Background: Unsafe water supplies continue to raise public health concerns, especially in urban areas in low resource countries.TounderstandtheextentofpublichealthriskattributedtosupplywaterinDhakacity,Bangladesh,Escherichia coli isolated from tap water samples collected from different locations of the city were characterized for their antibiotic resistance,pathogenicpropertiesandgeneticdiversity. Methodology/Principal Findings: A total of 233 E. coli isolates obtained from 175 tap water samples were analysed for susceptibilityto16differentantibioticsandforthepresenceofgenesassociatedwithvirulenceandantibioticresistance. Nearly36%(n=84)oftheisolatesweremulti-drug($3classesofantibiotics)resistant(MDR)and26%(n=22)ofthesewere positive for extended spectrum b-lactamase (ESBL). Of the 22 ESBL-producers, 20 were positive for blaCTX-M-15, 7 for blaOXA-1-group (all had blaOXA-47) and 2 for blaCMY-2. Quinolone resistance genes, qnrS and qnrB were detected in 6 and 2 isolates,respectively.Around7%(n=16)oftheisolatescarriedvirulencegene(s)characteristicofpathogenicE.coli;11of thesecontainedltand/orstandthusbelongedtoenterotoxigenicE.coliand5containedbfpandeaeandthusbelongedto enteropathogenicE.coli.AllMDRisolatescarriedmultipleplasmids(2to8)ofvaryingsizesrangingfrom1.2to.120MDa. Ampicillinandceftriaxoneresistancewereco-transferredinconjugativeplasmidsof7