Association of Duffy Blood Group Gene Polymorphisms with IL8 Gene in Chronic Periodontitis.docVIP

Association of Duffy Blood Group Gene Polymorphisms with IL8 Gene in Chronic Periodontitis Emília ?ngela Sippert , Cléverson de Oliveira e Silva , Jeane Eliete Laguila Visentainer , Ana Maria Sell 1 2 3 3* 1 Post Graduation Program of Biosciences Applied to Pharmacy, Department of Analysis Clinical and Biomedicine, Maringa State University, Parana, Brazil, 2 Dentistry Department, Maringa State University, Parana, Brazil, 3 Basic Health Sciences Department, Maringa State University, Parana, Brazil Abstract The antigens of the Duffy blood group system (DARC) act as a receptor for the interleukin IL-8. IL-8 plays an important role in the pathogenesis of chronic periodontitis due to its chemotactic properties on neutrophils. The aim of this study was to investigate a possible association of Duffy blood group gene polymorphisms with the -353TA, -845TC and -738TA SNPs of the IL8 gene in chronic periodontitis. One hundred and twenty-four individuals with chronic periodontitis and 187 controls were enrolled. DNA was extracted using the salting-out method. The Duffy genotypes and IL8 gene promoter polymorphisms were investigated by PCR-RFLP. Statistical analyses were conducted using the Chi square test with Yates correction or Fishers Exact Test, and the possibility of associations were evaluated by odds ratio with a 95% confidence interval. When analyzed separately, for the Duffy blood group system, differences in the genotype and allele frequencies were not observed between all the groups analyzed; and, in nonsmokers, the -845C allele (3.6% vs. 0.4%), -845TC genotype (7.3% vs. 0.7%) and the CTA haplotype (3.6% vs. 0.4%) were positively associated with chronic periodonti