AtEXP2 Is Involved in Seed Germination and Abiotic Stress Response in Arabidopsis.docVIP

AtEXP2IsInvolvedinSeedGerminationandAbiotic StressResponseinArabidopsis AnYan1,MinjieWu1,LimeiYan1,RuiHu2,ImranAli1,YinboGan1* 1ZhejiangKeyLabofCropGermplasm,DepartmentofAgronomy,CollegeofAgricultureandBiotechnology,ZhejiangUniversity,Hangzhou,Zhejiang,China,2Institute ofHydrobiology,ChineseAcademyofSciences,Wuhan,Hubei,China Abstract ExpansinsarecellwallproteinsthatpromotecellwalllooseningbyinducingpH-dependentcellwallextensionandstress relaxation. Expansins are required in a series of physiological developmental processes in higher plants such as seed germination.HereweidentifiedanArabidopsisexpansingeneAtEXPA2thatisexclusivelyexpressedingerminatingseeds andthemutantshowsdelayedgermination,suggestingthatAtEXP2isinvolvedincontrollingseedgermination.Exogenous GAapplicationincreasedtheexpressionlevelofAtEXP2duringseedgermination,whileABAapplicationhadnoeffecton AtEXP2expression.Furthermore,theanalysisofDELLAmutantsshowthatRGL1,RGL2,RGA,GAIareallinvolvedinrepressing AtEXP2expression,andRGL1playsthemostdominantroleincontrollingAtEXP2expression.Instressresponse,exp2mutant showshighersensitivitythanwildtypeinseedgermination,whileoverexpressionlinesofAtEXP2arelesssensitivetosalt stressandosmoticstress,exhibitingenhancedtolerancetostresstreatment.Collectively,ourresultssuggestthatAtEXP2is involvedintheGA-mediatedseedgerminationandconferssaltstressandosmoticstresstoleranceinArabidopsis. Citation:YanA,WuM,YanL,HuR,AliI,etal.(2014)AtEXP2IsInvolvedinSeedGerminationandAbioticStressResponseinArabidopsis.PLoSONE9(1):e85208. doi:10.1371/journal.pone.0085208 Editor:JohnSchiefelbein,UniversityofMichigan,UnitedStatesofAmerica ReceivedNovember3,2013;AcceptedNovember29,2013;PublishedJanuary3,2014 Copyright:?2014Yanetal.Thisisanopen-accessarticledistributedunderthetermsoftheCreativeCommonsAttributionLicense,whichpermitsunrestricted use,distribution,andreproductioninanymedium,providedtheoriginalauthorandsourcearecredited. Funding: Theresearch was supported byInternational Scientificand