Bacillus thuringiensis-derived Cry5B Has Potent Anthelmintic Activity against Ascaris suum.docVIP

下载本文档

2
0
约3.95万字
约 7页
2017-05-07 发布于上海
举报
版权申诉

Bacillus thuringiensis-derived Cry5B Has Potent Anthelmintic Activity against Ascaris suum.doc

1、原创力文档（book118）网站文档一经付费（服务费），不意味着购买了该文档的版权，仅供个人/单位学习、研究之用，不得用于商业用途，未经授权，严禁复制、发行、汇编、翻译或者网络传播等，侵权必究。。
2、本站所有内容均由合作方或网友上传，本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺！文档内容仅供研究参考，付费前请自行鉴别。如您付费，意味着您自己接受本站规则且自行承担风险，本站不退款、不进行额外附加服务；查看《如何避免下载的几个坑》。如果您已付费下载过本站文档，您可以点击这里二次下载。
3、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等，请点击“版权申诉”（推荐），也可以打举报电话：400-050-0827(电话支持时间：9:00-18:30)。
4、该文档为VIP文档，如果想要下载，成为VIP会员后，下载免费。
5、成为VIP后，下载本文档将扣除1次下载权益。下载后，不支持退款、换文档。如有疑问请联系我们。
6、成为VIP后，您将拥有八大权益，权益包括：VIP文档下载权益、阅读免打扰、文档格式转换、高级专利检索、专属身份标志、高级客服、多端互通、版权登记。
7、VIP文档为合作方或网友上传，每下载1次，网站将根据用户上传文档的质量评分、类型等，对文档贡献者给予高额补贴、流量扶持。如果你也想贡献VIP文档。上传文档

Bacillus thuringiensis-derived Cry5B Has Potent Anthelmintic Activity against Ascaris suum

Bacillusthuringiensis-derivedCry5BHasPotent AnthelminticActivityagainstAscarissuum JosephF.Urban,Jr.1.,YanHu2.,MelanieM.Miller2,UlrikeScheib2,YingY.Yiu2,RaffiV.Aroian2* 1USDA,AgriculturalResearchService, BeltsvilleHuman Nutrition ResearchCenter, Diet,Genomics,andImmunologyLaboratory, Beltsville, Maryland,United States of America,2SectionofCellandDevelopmentalBiology,DivisionofBiologicalSciences,UniversityofCalifornia,SanDiego,LaJolla,California,UnitedStatesofAmerica Abstract Ascaris suum and Ascaris lumbricoides are two closely related geo-helminth parasites that ubiquitously infect pigs and humans, respectively. Ascaris suum infection in pigs is considered agood model for A. lumbricoides infection in humans becauseofasimilarbiologyandtissuemigrationtotheintestines.Ascarislumbricoidesinfectionsinchildrenareassociated withmalnutrition,growthandcognitivestunting,immunedefects,and,inextremecases,life-threateningblockageofthe digestivetractandaberrantmigrationintothebileductandperitoneum.SimilareffectscanbeseenwithA.suuminfections inpigsrelatedtopoorfeedefficiencyandperformance.NewstrategiestocontrolAscarisinfectionsareneededlargelydue toreducedtreatmentefficaciesofcurrentanthelminticsinthefield,thethreatofresistancedevelopment,andthegeneral lackofnewdrugdevelopmentforintestinalsoil-transmittedhelminthsforhumansandanimals.Herewedemonstratefor thefirsttimethatA.suumexpressesthereceptorsforBacillusthuringiensiscrystalproteinandnovelanthelminticCry5B, whichhasbeenpreviouslyshowntointoxicatehookwormsandwhichbelongstoaclassofproteinsconsiderednon-toxic tovertebrates.Cry5BisabletointoxicateA.suumlarvaeandadultsandtriggerstheactivationofthep38mitogen-activated proteinkinasepathwaysimilartothatobservedwithothernematodes.Mostimportantly,twomoderatedosesof20mg/kg bodyweight(143nM/kg)ofCry5BresultedinanearcompletecureofintestinalA.suuminfectionsinpigs.Takentogether, theseresultsdemonstratetheexcellentpotentialofCry5BtotreatAscarisinfectionsinpigsandinhumansandforCry5Bto work