Bcl-2 Promoter Sequence G-Quadruplex Interactions with Three Planar and Non-Planar Cationic Porphyrins TMPyP4, TMPyP3, and TMPyP2.docVIP
- 1、原创力文档(book118)网站文档一经付费(服务费),不意味着购买了该文档的版权,仅供个人/单位学习、研究之用,不得用于商业用途,未经授权,严禁复制、发行、汇编、翻译或者网络传播等,侵权必究。。
- 2、本站所有内容均由合作方或网友上传,本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺!文档内容仅供研究参考,付费前请自行鉴别。如您付费,意味着您自己接受本站规则且自行承担风险,本站不退款、不进行额外附加服务;查看《如何避免下载的几个坑》。如果您已付费下载过本站文档,您可以点击 这里二次下载。
- 3、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等,请点击“版权申诉”(推荐),也可以打举报电话:400-050-0827(电话支持时间:9:00-18:30)。
- 4、该文档为VIP文档,如果想要下载,成为VIP会员后,下载免费。
- 5、成为VIP后,下载本文档将扣除1次下载权益。下载后,不支持退款、换文档。如有疑问请联系我们。
- 6、成为VIP后,您将拥有八大权益,权益包括:VIP文档下载权益、阅读免打扰、文档格式转换、高级专利检索、专属身份标志、高级客服、多端互通、版权登记。
- 7、VIP文档为合作方或网友上传,每下载1次, 网站将根据用户上传文档的质量评分、类型等,对文档贡献者给予高额补贴、流量扶持。如果你也想贡献VIP文档。上传文档
查看更多
Bcl-2 Promoter Sequence G-Quadruplex Interactions with Three Planar and Non-Planar Cationic Porphyrins TMPyP4, TMPyP3, and TMPyP2
Bcl-2 Promoter Sequence G-Quadruplex Interactions with
Three Planar and Non-Planar Cationic Porphyrins:
TMPyP4, TMPyP3, and TMPyP2
Vu H. Le , Narayana Nagesh , Edwin A. Lewis
1 2 1*
1 Department of Chemistry, Mississippi State University, Mississippi State, Mississippi, United States of America, 2 Centre for Cellular and Molecular Biology,
Hyderabad, Andhra Pradesh, India
Abstract
The interactions of three related cationic porphyrins, TMPyP4, TMPyP3 and TMPyP2, with a WT 39-mer Bcl-2
promoter sequence G-quadruplex were studied using Circular Dichroism, ESI mass spectrometry, Isothermal
Titration Calorimetry, and Fluorescence spectroscopy. The planar cationic porphyrin TMPyP4 (5, 10, 15, 20-meso-
tetra (N-methyl-4-pyridyl) porphine) is shown to bind to a WT Bcl-2 G-quadruplex via two different binding modes, an
end binding mode and a weaker mode attributed to intercalation. The related non-planar ligands, TMPyP3 and
TMPyP2, are shown to bind to the Bcl-2 G-quadruplex by a single mode. ESI mass spectrometry experiments
confirmed that the saturation stoichiometry is 4:1 for the TMPyP4 complex and 2:1 for the TMPyP2 and TMPyP3
complexes. ITC experiments determined that the equilibrium constant for formation of the (TMPyP4)1/DNA complex
(K1 = 3.7 × 10
6
) is approximately two orders of magnitude greater than the equilibrium constant for the formation of
). Porphyrin fluorescence is consistent with intercalation in the case of
the (TMPyP2)1/DNA complex, (K1 = 7.0 × 10
4
the (TMPyP4)3/DNA and (TMPyP4)4/DNA complexes. The non-planar shape of the TMPyP2 and TMPyP3 molecules
results in both a reduced affinity for the end binding interaction and the elimination of the intercalation binding mode.
Citation: Le VH, Nagesh N, Lewis EA (2013) Bcl-2 Promoter Sequence G-Quadruplex Interactions with Three
您可能关注的文档
- Bacteriophage Receptor Binding Protein Based Assays for the Simultaneous Detection of Campylobacter jejuni and Campylobacter coli.doc
- Baculovirus as an Ideal Radionuclide Reporter Gene Vector A New Strategy for Monitoring the Fate of Human Stem Cells In Vivo.doc
- Bad Taste Protects Fruit Flies from Eating a Toxic Amino Acid in Plants.doc
- Baculovirus Superinfection A Probable Restriction Factor on the Surface Display of Proteins for Library Screening.doc
- Baculoviruses Sophisticated Pathogens of Insects.doc
- Bacteriuria amongst Pregnant Women in the Buea Health District, Cameroon Prevalence, Predictors, Antibiotic Susceptibility Patterns and Diagnosis.doc
- BAFF Receptor mAb Treatment Ameliorates Development and Progression of Atherosclerosis in Hyperlipidemic ApoE?? Mice.doc
- BAFF Controls Neural Cell Survival through BAFF Receptor.doc
- Bacteriophages in.doc
- Bacterial Inclusion Bodies Contain Amyloid-Like Structure.doc
- BCL-3 Attenuation of TNFA Expression Involves an Incoherent Feed-Forward Loop Regulated by Chromatin Structure.doc
- Bcl-XL Inhibits Membrane Permeabilization by Competing with Bax.doc
- Bayesian Modeling of the Yeast SH3 Domain Interactome Predicts Spatiotemporal Dynamics of Endocytosis Proteins.doc
- BcL-xL Conformational Changes upon Fragment Binding Revealed by NMR.doc
- Bcl-w Enhances Mesenchymal Changes and Invasiveness of Glioblastoma Cells by Inducing Nuclear Accumulation of β-Catenin.doc
- Bcl-xL Silencing Induces Alterations in hsa-miR-608 Expression and Subsequent Cell Death in A549 and SK-LU1 Human Lung Adenocarcinoma Cells.doc
- BCRPABCG2 Inhibition Sensitizes Hepatocellular Carcinoma Cells to Sorafenib.doc
- Bayesian Parameter Inference by Markov Chain Monte Carlo with Hybrid Fitness Measures Theory and Test in Apoptosis Signal Transduction Network.doc
- BDNF Depresses Excitability of Parvalbumin-Positive Interneurons through an M-Like Current in Rat Dentate Gyrus.doc
- BDNF Val66Met Variant and Smoking in a Chinese Population.doc
文档评论(0)