Characterization of the Two Intra-Individual Sequence Variants in the 18S rRNA Gene in the Plant Parasitic Nematode, Rotylenchulus renormis.docVIP

下载本文档

5
0
约5.03万字
约 13页
2017-05-07 发布于上海
举报
版权申诉

Characterization of the Two Intra-Individual Sequence Variants in the 18S rRNA Gene in the Plant Parasitic Nematode, Rotylenchulus renormis.doc

1、原创力文档（book118）网站文档一经付费（服务费），不意味着购买了该文档的版权，仅供个人/单位学习、研究之用，不得用于商业用途，未经授权，严禁复制、发行、汇编、翻译或者网络传播等，侵权必究。。
2、本站所有内容均由合作方或网友上传，本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺！文档内容仅供研究参考，付费前请自行鉴别。如您付费，意味着您自己接受本站规则且自行承担风险，本站不退款、不进行额外附加服务；查看《如何避免下载的几个坑》。如果您已付费下载过本站文档，您可以点击这里二次下载。
3、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等，请点击“版权申诉”（推荐），也可以打举报电话：400-050-0827(电话支持时间：9:00-18:30)。
4、该文档为VIP文档，如果想要下载，成为VIP会员后，下载免费。
5、成为VIP后，下载本文档将扣除1次下载权益。下载后，不支持退款、换文档。如有疑问请联系我们。
6、成为VIP后，您将拥有八大权益，权益包括：VIP文档下载权益、阅读免打扰、文档格式转换、高级专利检索、专属身份标志、高级客服、多端互通、版权登记。
7、VIP文档为合作方或网友上传，每下载1次，网站将根据用户上传文档的质量评分、类型等，对文档贡献者给予高额补贴、流量扶持。如果你也想贡献VIP文档。上传文档

Characterization of the Two Intra-Individual Sequence Variants in the 18S rRNA Gene in the Plant Parasitic Nematode, Rotylenchulus renormis

CharacterizationoftheTwoIntra-IndividualSequence Variantsinthe18SrRNAGeneinthePlantParasitic Nematode,Rotylenchulusreniformis SeloameT.Nyaku1*,VenkateswaraR.Sripathi1,RameshV.Kantety1,YongQ.Gu2,KathyLawrence3, GovindC.Sharma1 1DepartmentofBiologicalandEnvironmentalSciences,AlabamaAMUniversity,Normal,Alabama,UnitedStatesofAmerica,2GenomicsandGeneDiscoveryResearch Unit,USDA-ARS,Albany,California,UnitedStatesofAmerica,3DepartmentofEntomologyandPlantPathology,AuburnUniversity,Auburn,Alabama,UnitedStatesof America Abstract The 18S rRNA gene is fundamental to cellular and organismal protein synthesis and because of its stable persistence throughgenerationsitisalsousedinphylogeneticanalysisamongtaxa.Sequencevariationinthisgenewithinasingle speciesisrare,butithasbeenobservedinfewmetazoanorganisms.Morefrequentlyithasmostlybeenreportedinthe non-transcribedspacer region.Here, wehaveidentifiedtwosequencevariantswithin thenearfullcoding regionof 18S rRNA gene from a single reniform nematode (RN) Rotylenchulus reniformis labeled as reniform nematode variant 1 (RN_VAR1)andvariant2(RN_VAR2).AllsequencesfromthreeofthefourisolateshadbothRNvariantsintheirsequences; however,isolate13BhadonlyRNvariant2sequence.Specificvariablebasesites(96or5.5%)werefoundwithinthe18S rRNAgenethatcanclearlydistinguishthetwo18SrDNAvariantsofRN,in11(25.0%)and33(75.0%)ofthe44RNclones,for RN_VAR1 and RN_VAR2, respectively. Neighbor-joining trees show that the RN_VAR1 is very similar to the previously existingR.reniformissequenceinGenBank,whiletheRN_VAR2sequenceismoredivergent.Thisisthefirstreportofthe identificationoftwomajorvariantsofthe18SrRNAgeneinthesamesingleRN,anddocumentsthespecificbasevariation betweenthetwovariants,andhypothesizesonsimultaneousco-existenceofthesetwovariantsforthisgene. Citation:NyakuST,SripathiVR,KantetyRV,GuYQ,LawrenceK,etal.(2013)CharacterizationoftheTwoIntra-IndividualSequenceVariantsinthe18SrRNAGene inthePlantParasiticNematode,Rotylenchulusreniformis.PLoSONE8(4):e60891.do