Visualization of protein interactions in living Drosophila embryos by the bimolecular fluorescence complementation assay 英文论文文献.docVIP

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Visualization of protein interactions in living Drosophila embryos by the bimolecular fluorescence complementation assay 英文论文文献.doc

Visualization of protein interactions in living Drosophila embryos by the bimolecular fluorescence complementation assay 英文论文文献

Hudryetal.BMCBiology2011,9:5 /1741-7007/9/5 METHODOLOGY ARTICLE OpenAccess Visualizationofproteininteractionsinliving Drosophilaembryosbythebimolecular fluorescencecomplementationassay BrunoHudry,SéverineViala,YacineGraba,SamirMerabet* Abstract Background:Proteininteractionscontroltheregulatorynetworksunderlyingdevelopmentalprocesses.The understandingofdevelopmentalcomplexitywill,therefore,requirethecharacterizationofproteininteractions withintheirproperenvironment.Thebimolecularfluorescencecomplementation(BiFC)technologyoffersthis possibilityasitenablesthedirectvisualizationofproteininteract

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