胺基裂解酶及其在医药中间体生产中的应用.PDFVIP

微生物学通报 JUL 20, 2008, 35(7): 1113~1118 Microbiology © 2008 by Institute of Microbiology, CAS tongbao@ 专论与综述 胺基裂解酶及其在医药中间体生产中的应用 何碧波 陈小龙 郑裕国* 沈寅初 ( 310032) 摘 要: C-N 裂解酶(E.C.4.3)是能催化释放氨、脒和胺基等并形成双键或环的一类酶。胺基裂解酶 (E.C.4.3.3)就是其中能够催化释放胺基的一类酶, 该类酶在医药中间体生产中起关键酶的作用。本 文概述了4 种胺基裂解酶的来源, 酶学特性以及它们在医药中间体生产中的应用。 关键词: 胺基裂解酶, C-N 裂解酶, 井冈霉素, 单萜吲哚生物碱 Amine-lyases and Their Applications in Preparation of Pharmaceutical Intermediates * HE Bi-Bo CHEN Xiao-Long ZHENG Yu-Guo SHEN Yin-Chu (Institute of Bioengineering, Zhejiang University of Technology, Hangzhou 310032) Abstract: Carbon-nitrogen lyases (E.C.4.3) are a group of enzymes that release ammonia, amidine or amino group etc, and also show ability to form double bond or ring structure. Specifically, enzymes forming amino group are called amine-lyases (E.C.4.3.3), which are critical in the industrial production of many medicine intermediates. In this review is a summary of four major amine-lyases in terms of their source, enzymatic characteristics and their applications in preparation of pharmaceutical intermediates. Keywords: Amine-lyases, C-N lyases, Validamycin, Monoterpenoid indole alkaloids C-N C-N , , (EC 4.3.1 Ammonia-lyases), , (EC 4.3.2 Amidine-lyases), (EC 4.3.3 Amine-lyases), C-N (EC 4.3.99 , Other Carbon-nitrogen lyases) 1 3-酮-井冈羟胺A C-N 裂解酶 3- - A C-N (3-Ketovali- doxylamine A C-N lyase)(strictosidine 3-- A C-N