长春花细胞生物碱合成培养过程的营养消耗研究.docVIP

Bioprocess 生物过程, 2012, 2, 58-64 /10.12677/bp.2012.22010 Published Online June 2012 (/journal/bp) The Study of Nutrient Consumption on Catharanthus roseus Cell in the Alkaloid Biosynthesis Culture Nan Zhao, Wen Chen, Zhigang Guo Department of Chemical Engineering, Tsinghua University, Beijing Email: guozhig@ Received: May 9th, 2012; revised: May 24th, 2012; accepted: Jun. 2nd, 2012 Abstract: Catharanthus roseus cells were cultured using the EPC-500 PE bio-fermenter, and a stirring speed of 150 r/min and gas supply of 0.5 mL/min were determined to be the best cultivating conditions