基于SPR干涉成像传感法检测大蒜素刺激胃癌细胞的响应.pdfVIP

第27卷 第4期 传 感 技 术 学 报 Vo1．27 No．4 2014年 4月 CHINESEJOURNALOFSENSORSANDACTUATORS Apr．2014 Detection ofLivingGastricCancerCellResponsetoAllicin Stimulation Basedon SurfacePlasmonResonanceInterferometricImagingSensing DOUFuyin， ⅣGPeng ，删 GRui，D删 GShijie，LVYouyong， Xinglong (1．StateKeyLaboratoryofPrecisionMeasurementTechnologyandInstruments，DepartmentofPrecsiionInstruments， TsinghuaUniversity，Beijing100084，China； 2LaboratoryofMolecularOncology，KeyLaboratoryofCarcinogenesisandTranslationalResearch(MinistryofEducation)， PekingUniversitySchoolofOneology，BeijingCancerHospitalandInstitute，Beijing100142，China) Abstract：Aimedattherequirementofliving-celldetection，themethodofSPR interferometricimagingsensingcell responsewasproposed．A novelmicrofluidicchamberwasdesigned，andthesystem usedformoleculardetectionon living—celllevelwassetup．Thecellcultured stateinchamberwasdetectedandanalyzed，andthentheresponseof gastriccancercelllineBGC823simulatedbyallicinwasmonitored．Theresultsshow thatgastriccancercellsadhere andgrow well，andthemicrofluidicchambercouldmaintaincellsurvivalmicroenvironment．TheSPR signalofcells stimulatedandnotstimulatedbyallicinwasrespectively0．15RIRF and0．35RIRF，which indicatedthatallicin hasobvious inhibitory effecton the cells．Itsuggeststhepossibility ofthe establishmentofa detection drug— stimulating—cellsplatform andcontributiontothedurgdevelopmentandclinicalchemotherapyaftertheprecision，re— liability，relativetechnologyarefurtherenhanced． Keywords：SPR interferometric imaging，microfluidic chamber，living—celldetection，allicin，gastric cancercell lineBGC823 EEACC：7230J doi：10．3969／j．issn．1004-1699．2014．04．003 基于SPR干涉成像传感法检测大蒜素刺激胃癌细胞的响应术 窦福印 ，王 鹏 ，邢 蕊 ，邓士杰 ，吕有勇 ，余兴龙 (1．清华大学精密仪器系精密测试技术及仪器国家重点实验室，北京 100084； 2．北京大学肿瘤医院／北京市肿瘤防治研究所恶性